ArticleScientific reports2018
Selection of suitable reference genes for qRT-PCR normalisation under different experimental conditions in Eucommia ulmoides Oliv.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
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- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- Cloning and functional characterization of the peptide deformylase encoding gene EuPDF1B from Eucommia ulmoides Oliv.Scientific reports · 2024Article
- StCoExpNet: a global co-expression network analysis facilitates identifying genes underlying agronomic traits in potatoes.Plant cell reports · 2024Article
- Selection of suitable reference lncRNAs for gene expression analysis inFrontiers in plant science · 2024Article
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- Ontogenetic Variation in Macrocyclic and Hemicyclic Poplar Rust Fungi.International journal of molecular sciences · 2022Article
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- Selection and validation of reference genes for quantitative real-time PCR of Quercus mongolica Fisch. ex Ledeb under abiotic stresses.PloS one · 2022Article
- Prognostic value ofFrontiers in genetics · 2022Article
- Selection and validation of reference genes for RT-qPCR analysis of different organs at various development stages inOpen life sciences · 2022Article
- Selection and Verification of Appropriate Reference Genes for Expression Normalization inGenes · 2021Article
- Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions.Scientific reports · 2021Article
- Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples.PloS one · 2021Article
- Multinuclei Occurred Under Cryopreservation and Enhanced the Pathogenicity ofFrontiers in microbiology · 2021Article
- Selection and validation of reliable reference genes for gene expression studies from Monilinia vaccinii-corymbosi infected wild blueberry phenotypes.Scientific reports · 2020Article
- Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf.Molecular biology reports · 2020Article
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Authors and funding
8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Normalisation of data, by choosing the appropriate reference genes, is fundamental for obtaining reliable results in quantitative real-time PCR (qPCR). This study evaluated the expression stability of 11 candidate reference genes with different varieties, developmental periods, tissues, and abiotic stresses by using four statistical algorithms: geNorm, NormFinder, BestKeeper, and RefFinder. The results indicated that ubiquitin-conjugating enzyme S (UBC) and ubiquitin-conjugating enzyme E2 (UBC E2) could be used as reference genes for different E. ulmoides varieties and tissues, UBC and histone H4 (HIS4) for different developmental periods, beta-tubulin (TUB) and UBC for cold treatment, ubiquitin extension protein (UBA80) and HIS4 for drought treatment, and ubiquitin-60S ribosomal protein L40 (UBA52) and UBC E2 for salinity treatment. UBC and UBC E2 for the group "Natural growth" and "Total", UBA80 and UBC for the group "Abiotic stresses". To validate the suitability of the selected reference genes in this study, mevalonate kinase (MK), phenylalanine ammonia-lyase (PAL), and 4-coumarate-CoA ligase (4CL) gene expression patterns were analysed. When the most unstable reference genes were used for normalisation, the expression patterns had significant biases compared with the optimum reference gene combinations. These results will be beneficial for more accurate quantification of gene expression levels in E. ulmoides.
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