ArticleScientific reports2018
Understanding the transfer reaction network behind the non-processive synthesis of low molecular weight levan catalyzed by Bacillus subtilis levansucrase.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 56 citations in OpenAlex.
- Process optimization and characterization of levan from Sporosarcina globispora MTCC 4776.Scientific reports · 2026Article
- Recent Advances in Microbial Levan Production: Strategies for Yield Enhancement and Sustainability.International journal of food science · 2026Review
- Disaccharides and Fructooligosaccharides (FOS) Production by Wild Yeasts Isolated from Agave.Foods (Basel, Switzerland) · 2025Article
- Valorization of Riceberry Broken Rice and Soybean Meal for Optimized Production of Multifunctional Exopolysaccharide byPolymers · 2025Article
- Molecular Basis ofJournal of agricultural and food chemistry · 2025Article
- A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases.Biomolecules · 2024Article
- Tailoring the natural rare sugars D-tagatose and L-sorbose to produce novel functional carbohydrates.NPJ science of food · 2024Article
- Neokestose suppresses the increase in plasma glucose caused by oral administration of sucrose in a streptozotocin‑induced diabetic rat.Scientific reports · 2024Article
- Characterization of Levan Fructan Produced by aMicroorganisms · 2024Article
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- Combinatorial metabolic engineering enables high yield production of α-arbutin from sucrose by biocatalysis.Applied microbiology and biotechnology · 2023Article
- Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pastoris strain expressing a levansucrase-endolevanase fusion enzyme.Microbial cell factories · 2023Article
- Levansucrase: Enzymatic Synthesis of Engineered Prebiotics.Current pharmaceutical biotechnology · 2023Article
- Erwinia tasmaniensis levansucrase shows enantiomer selection for (S)-1,2,4-butanetriol.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Separation of Fructosyl Oligosaccharides in Maple Syrup by Using Charged Aerosol Detection.Foods (Basel, Switzerland) · 2021Article
- Article
- Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.PloS one · 2021Article
- Correction: Sato, K., et al. Identification of a Novel Oligosaccharide in Maple Syrup as a Potential Alternative Saccharide for Diabetes Mellitus Patients.International journal of molecular sciences · 2020Article
- Levansucrase fromBiomolecules · 2020Article
- The Structure of Sucrose-Soaked Levansucrase Crystals fromInternational journal of molecular sciences · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Under specific reaction conditions, levansucrase from Bacillus subtilis (SacB) catalyzes the synthesis of a low molecular weight levan through the non-processive elongation of a great number of intermediates. To deepen understanding of the polymer elongation mechanism, we conducted a meticulous examination of the fructooligosaccharide profile evolution during the levan synthesis. As a result, the formation of primary and secondary intermediates series in different reaction stages was observed. The origin of the series was identified through comparison with product profiles obtained in acceptor reactions employing levanbiose, blastose, 1-kestose, 6-kestose, and neo-kestose, and supported with the isolation and NMR analyses of some relevant products, demonstrating that all of them are inherent products during levan formation from sucrose. These results allowed to establish the network of fructosyl transfer reactions involved in the non-processive levan synthesis. Overall, our results reveal how the relaxed acceptor specificity of SacB during the initial steps of the synthesis is responsible for the formation of several levan series, which constitute the final low molecular weight levan distribution.
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