Evidence map›Paper›PMID 30301888›Full record

ArticleNature communications2018

Microglia innately develop within cerebral organoids.

Paul R Ormel, Renata Vieira de Sá, Emma J van Bodegraven, Henk Karst, Oliver Harschnitz, Marjolein A M Sneeboer, Lill Eva Johansen, Roland E van Dijk, Nicky Scheefhals, Amber Berdenis van Berlekom and 8 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 392 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
392citing papers in PubMed, 5 pooled it
25.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

392 citing papers in PubMed, 5 syntheses or guidelines pooled it, 609 citations in OpenAlex.

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332 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors at 2 institutions in 1 country.

Paul R OrmelDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Renata Vieira de SáDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Emma J van BodegravenDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Henk KarstDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Oliver HarschnitzDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0001-6496-881X
Marjolein A M SneeboerDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Lill Eva JohansenDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Roland E van DijkDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Nicky ScheefhalsCell Biology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
Amber Berdenis van BerlekomDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Eduardo Ribes MartínezDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Sandra KlingDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Harold D MacGillavryCell Biology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-6153-3586
Leonard H van den BergDepartment of Neurology, BCRM-UMCU, Utrecht University, P.O. Box 85500, Heidelberglaan 100, 3508 GA, Utrecht, The Netherlands.
René S KahnDepartment of Psychiatry, BCRM-UMCU, Utrecht University, P.O. Box 85500, Heidelberglaan 100, 3508 GA, Utrecht, The Netherlands.
Elly M HolDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
Lot D de WitteDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands. L.D.deWitte@umcutrecht.nl.
R Jeroen PasterkampDepartment of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht (BCRM-UMCU), Utrecht University, P.O. Box 85500, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0003-1631-6440
Utrecht University · NLNetherlands Institute for Neuroscience · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cerebral organoids are 3D stem cell-derived models that can be utilized to study the human brain. The current consensus is that cerebral organoids consist of cells derived from the neuroectodermal lineage. This limits their value and applicability, as mesodermal-derived microglia are important players in neural development and disease. Remarkably, here we show that microglia can innately develop within a cerebral organoid model and display their characteristic ramified morphology. The transcriptome and response to inflammatory stimulation of these organoid-grown microglia closely mimic the transcriptome and response of adult microglia acutely isolated from post mortem human brain tissue. In addition, organoid-grown microglia mediate phagocytosis and synaptic material is detected inside them. In all, our study characterizes a microglia-containing organoid model that represents a valuable tool for studying the interplay between microglia, macroglia, and neurons in human brain development and disease.

Indexed as

AdultAgedAged, 80 and overCerebrumFemaleGerm LayersHumansImmunityMaleMesodermMicrogliaMiddle AgedNeuronsOrganoidsStem CellsTranscriptome

Identifiers

PMID30301888
PMCPMC6177485
OpenAlexW2895538826

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.