ArticlemBio2018
Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages.
Article in mBio, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed.
- Capsular Polysaccharide Safeguards a Prophage-Bacterium Symbiosis by Preventing Collateral Attack and Promoting Viral Transmission.bioRxiv : the preprint server for biology · 2026Article
- Geometric ordering in bacterial communities.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Spatial microenvironments tune immune response dynamics in the Drosophila larval fat body.PLoS genetics · 2026Article
- Imaging the impact of rotifer consumption on bacterial behaviors in the zebrafish gut.PloS one · 2026Article
- Opposing roles for iron transport systems in gallium tolerance in extraintestinal pathogenicJournal of bacteriology · 2025Article
- Spatial microenvironments tune immune response dynamics in thebioRxiv : the preprint server for biology · 2025Article
- Characterizing trajectories of innate immune cells in larval zebrafish.bioRxiv : the preprint server for biology · 2025Article
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- Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors.Veterinary research · 2025Article
- Phollow reveals in situ phage transmission dynamics in the zebrafish gut microbiome at single-virion resolution.Nature microbiology · 2025Article
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- ThemBio · 2025Article
- A strong priority effect in the assembly of a specialized insect-microbe symbiosis.Applied and environmental microbiology · 2024Article
- Structural idiosyncrasies of glycyl T-box riboswitches among pathogenic bacteria.RNA (New York, N.Y.) · 2024Article
- Phollow: Visualizing Gut Bacteriophage Transmission within Microbial Communities and Living Animals.bioRxiv : the preprint server for biology · 2024Article
- Article
- Bacterial metallothionein, PmtA, a novel stress protein found on the bacterial surface ofmSphere · 2024Article
- Ecological drift during colonization drives within-host and between-host heterogeneity in an animal-associated symbiont.PLoS biology · 2024Article
- Metabolic model predictions enable targeted microbiome manipulation through precision prebiotics.Microbiology spectrum · 2024Article
- Genes associated with fitness and disease severity in the pan-genome of mastitis-associatedFrontiers in microbiology · 2024Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Correlating the presence of bacteria and the genes they carry with aspects of plant and animal biology is rapidly outpacing the functional characterization of naturally occurring symbioses. A major barrier to mechanistic studies is the lack of tools for the efficient genetic manipulation of wild and diverse bacterial isolates. To address the need for improved molecular tools, we used a collection of proteobacterial isolates native to the zebrafish intestinal microbiota as a testbed to construct a series of modernized vectors that expedite genetic knock-in and knockout procedures across lineages. The innovations that we introduce enhance the flexibility of conventional genetic techniques, making it easier to manipulate many different bacterial isolates with a single set of tools. We developed alternative strategies for domestication-free conjugation, designed plasmids with customizable features, and streamlined allelic exchange using visual markers of homologous recombination. We demonstrate the potential of these tools through a comparative study of bacterial behavior within the zebrafish intestine. Live imaging of fluorescently tagged isolates revealed a spectrum of distinct population structures that differ in their biogeography and dominant growth mode (i.e., planktonic versus aggregated). Most striking, we observed divergent genotype-phenotype relationships: several isolates that are predicted by genomic analysis and
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.