ArticleJournal of cell science2018
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis.
Article in Journal of cell science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Alterations of actin in aging.Journal of cell science · 2026Review
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- JAGGED1 modulates bull spermatozoa acrosome reaction and fertilization.Reproduction & fertility · 2026Article
- Real-Time Acrosome Reaction and Intracellular Calcium Recordings in Mice Sperm.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cryopreservation of Yak Semen: A Comprehensive Review.Animals : an open access journal from MDPI · 2022Review
- Extending resolution within a single imaging frame.Nature communications · 2022Article
- The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.The Journal of biological chemistry · 2022Article
- Mysteries and unsolved problems of mammalian fertilization and related topics.Biology of reproduction · 2022Review
- Quantitative phosphoproteomics analyses reveal the regulatory mechanisms related to frozen-thawed sperm capacitation and acrosome reaction in yak (Frontiers in physiology · 2022Article
- Discrete Dynamic Model of the Mammalian Sperm Acrosome Reaction: The Influence of Acrosomal pH and Physiological Heterogeneity.Frontiers in physiology · 2021Article
- Sperm Differentiation: The Role of Trafficking of Proteins.International journal of molecular sciences · 2020Review
- Cryo-EM Structure of Actin Filaments fromThe Plant cell · 2019Article
- Advances in Cryopreservation of Bull Sperm.Frontiers in veterinary science · 2019Review
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Authors and funding
15 authors at 3 institutions in 3 countries.
Funding
Abstract
Filamentous actin (F-actin) is a key factor in exocytosis in many cell types. In mammalian sperm, acrosomal exocytosis (denoted the acrosome reaction or AR), a special type of controlled secretion, is regulated by multiple signaling pathways and the actin cytoskeleton. However, the dynamic changes of the actin cytoskeleton in live sperm are largely not understood. Here, we used the powerful properties of SiR-actin to examine actin dynamics in live mouse sperm at the onset of the AR. By using a combination of super-resolution microscopy techniques to image sperm loaded with SiR-actin or sperm from transgenic mice containing Lifeact-EGFP, six regions containing F-actin within the sperm head were revealed. The proportion of sperm possessing these structures changed upon capacitation. By performing live-cell imaging experiments, we report that dynamic changes of F-actin during the AR occur in specific regions of the sperm head. While certain F-actin regions undergo depolymerization prior to the initiation of the AR, others remain unaltered or are lost after exocytosis occurs. Our work emphasizes the utility of live-cell nanoscopy, which will undoubtedly impact the search for mechanisms that underlie basic sperm functions.This article has an associated First Person interview with the first author of the paper.
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Registered trials
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