Evidence map›Paper›PMID 30300966›Full record

ArticleChembiochem : a European journal of chemical biology2018

Cucurbit[8]uril Reactivation of an Inactivated Caspase-8 Mutant Reveals Differentiated Enzymatic Substrate Processing.

Dung T Dang, Arthur H A M van Onzen, Yvonne L Dorland, Luc Brunsveld

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Dung T DangLaboratory of Chemical Biology, Department of Biomedical Engineering, and, Institute for Complex Molecular Systems, Eindhoven University of Technology, Den Dolech 2, 5612, AZ, Eindhoven, The Netherlands.
Arthur H A M van OnzenLaboratory of Chemical Biology, Department of Biomedical Engineering, and, Institute for Complex Molecular Systems, Eindhoven University of Technology, Den Dolech 2, 5612, AZ, Eindhoven, The Netherlands.
Yvonne L DorlandLaboratory of Chemical Biology, Department of Biomedical Engineering, and, Institute for Complex Molecular Systems, Eindhoven University of Technology, Den Dolech 2, 5612, AZ, Eindhoven, The Netherlands.
Luc BrunsveldLaboratory of Chemical Biology, Department of Biomedical Engineering, and, Institute for Complex Molecular Systems, Eindhoven University of Technology, Den Dolech 2, 5612, AZ, Eindhoven, The Netherlands.ORCID 0000-0001-5675-511X
Eindhoven University of Technology · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Caspase-8 constructs featuring an N-terminal FGG sequence allow for selective twofold recognition by cucurbit[8]uril, which leads to an increase of the enzymatic activity in a cucurbit[8]uril dose-dependent manner. This supramolecular switching has enabled for the first time the study of the same caspase-8 in its two extreme states; as full monomer and as cucurbit[8]uril induced dimer. A mutated, fully monomeric caspase-8 (D384A), which is enzymatically inactive towards its natural substrate caspase-3, could be fully reactivated upon addition of cucurbit[8]uril. In its monomeric state caspase-8 (D384A) still processes a small synthetic substrate, but not the natural caspase-3 substrate, highlighting the close interplay between protein dimerization and active site rearrangement for substrate selectivity. The ability to switch the caspase-8 activity by a supramolecular system thus provides a flexible approach to studying the activity of a protein at different oligomerization states.

Indexed as

Bridged-Ring CompoundsCaspase 8CatalysisCucurbit(n)urilsEnzyme ReactivatorsHumansImidazolesMacrocyclic CompoundsPoint MutationProtein MultimerizationBridged-Ring CompoundsCASP8 protein, humanCaspase 8cucurbit(8)urilCucurbit(n)urilsEnzyme ReactivatorsImidazolesMacrocyclic Compoundscaspasescucurbit[8]urilprotein assemblyprotein engineeringsupramolecular chemistry

Identifiers

PMID30300966
PMCPMC6391946
OpenAlexW2897205486

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.