ArticleThe FEBS journal2018
Biochemical characterization of the minimal domains of an iterative eukaryotic polyketide synthase.
Article in The FEBS journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
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- An endosymbiotic origin of the crimson pigment from the lac insect.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Interpretable adenylation domain specificity prediction using protein language models.bioRxiv : the preprint server for biology · 2025Article
- Six type-I PKS classes and highly conserved melanin and elsinochrome gene clusters found in diverse Elsinoë species.BMC genomics · 2024Article
- Chromosome-level genome assembly of the sacoglossan sea slug Elysia timida (Risso, 1818).BMC genomics · 2024Article
- Genome Mining ofMicroorganisms · 2024Article
- Advances in Aureobasidium research: Paving the path to industrial utilization.Microbial biotechnology · 2024Review
- Transcriptomic Response of the Atlantic Surfclam (Spisula solidissima) to Acute Heat Stress.Marine biotechnology (New York, N.Y.) · 2024Article
- Biosynthesis of the Isocoumarin Derivatives Fusamarins is Mediated by the PKS8 Gene Cluster in Fusarium.Chembiochem : a European journal of chemical biology · 2023Article
- Linking Lichen Metabolites to Genes: Emerging Concepts and Lessons from Molecular Biology and Metagenomics.Journal of fungi (Basel, Switzerland) · 2023Review
- Genomic Analysis of theMycobiology · 2023Article
- The mud-dwelling clamProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Diverse host-associated fungal systems as a dynamic source of novel bioactive anthraquinones in drug discovery: Current status and future perspectives.Journal of advanced research · 2022Review
- Mining genomes to illuminate the specialized chemistry of life.Nature reviews. Genetics · 2021Review
- Biosynthesis of Fusapyrone Depends on the H3K9 Methyltransferase, FmKmt1, inFrontiers in fungal biology · 2021Article
- Animal biosynthesis of complex polyketides in a photosynthetic partnership.Nature communications · 2020Article
- The biosynthetic diversity of the animal world.The Journal of biological chemistry · 2019Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Iterative type I polyketide synthases (PKS) are megaenzymes essential to the biosynthesis of an enormously diverse array of bioactive natural products. Each PKS contains minimally three functional domains, β-ketosynthase (KS), acyltransferase (AT), and acyl carrier protein (ACP), and a subset of reducing domains such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER). The substrate selection, condensation reactions, and β-keto processing of the polyketide growing chain are highly controlled in a programmed manner. However, the structural features and mechanistic rules that orchestrate the iterative cycles, processing domains functionality, and chain termination in this kind of megaenzymes are often poorly understood. Here, we present a biochemical and functional characterization of the KS and the AT domains of a PKS from the mallard duck Anas platyrhynchos (ApPKS). ApPKS belongs to an animal PKS family phylogenetically more related to bacterial PKS than to metazoan fatty acid synthases. Through the dissection of the ApPKS enzyme into mono- to didomain fragments and its reconstitution in vitro, we determined its substrate specificity toward different starters and extender units. ApPKS AT domain can effectively transfer acetyl-CoA and malonyl-CoA to the ApPKS ACP stand-alone domain. Furthermore, the KS and KR domains, in the presence of Escherichia coli ACP, acetyl-CoA, and malonyl-CoA, showed the ability to catalyze the chain elongation and the β-keto reduction steps necessary to yield a 3-hydroxybutyryl-ACP derivate. These results provide new insights into the catalytic efficiency and specificity of this uncharacterized family of PKSs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.