ArticleAnalytical chemistry2018
Liquid Chromatography-Tandem Mass Spectrometry Approach for Determining Glycosidic Linkages.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 79 citations in OpenAlex.
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- Glycoside hydrolase-mediated glucomannan catabolism inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Robust and High-Resolution All-Ion Fragmentation LC-ESI-IM-MS Analysis for In-Depth Characterization or Profiling of Up to 200 Human Milk Oligosaccharides.Analytical chemistry · 2025Article
- A multi-glycomic platform for the analysis of food carbohydrates.Nature protocols · 2024Review
- Characterization of the exopolysaccharides produced by the industrial yeast Komagataella phaffii.Journal of industrial microbiology & biotechnology · 2024Article
- Effects of Blanching, Freezing and Canning on the Carbohydrates in Sweet Corn.Foods (Basel, Switzerland) · 2023Article
- Two-dimensional isomer differentiation using liquid chromatography-tandem mass spectrometry with in-source, droplet-based derivatization.The Analyst · 2023Article
- Analysis of Cell Glycogen with Quantitation and Determination of Branching Using Liquid Chromatography-Mass Spectrometry.Analytical chemistry · 2023Article
- Multi-Glycomic Characterization of Fiber from AOAC Methods Defines the Carbohydrate Structures.Journal of agricultural and food chemistry · 2022Article
- Fucoidan from Marine Macroalgae: Biological Actions and Applications in Regenerative Medicine, Drug Delivery Systems and Food Industry.Bioengineering (Basel, Switzerland) · 2022Review
- N-Glycans in Immortalized Mesenchymal Stromal Cell-Derived Extracellular Vesicles Are Critical for EV-Cell Interaction and Functional Activation of Endothelial Cells.International journal of molecular sciences · 2022Article
- Biocatalytic quantification of α-glucan in marine particulate organic matter.MicrobiologyOpen · 2022Article
- An approach for evaluating the effects of dietary fiber polysaccharides on the human gut microbiome and plasma proteome.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural Elucidation and Activities ofFrontiers in nutrition · 2022Review
- A Multidimensional Mass Spectrometry-Based Workflow forJournal of the American Society for Mass Spectrometry · 2021Article
- Strain-level functional variation in the human gut microbiota based on bacterial binding to artificial food particles.Cell host & microbe · 2021Article
- Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues.Biotechnology for biofuels · 2021Review
- Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.PloS one · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The structural analysis of carbohydrates remains challenging mainly due to the lack of rapid analytical methods able to determine and quantitate glycosidic linkages between the diverse monosaccharides found in natural oligosaccharides and polysaccharides. In this research, we present the first liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for the rapid and simultaneous relative quantitation of glycosidic linkages for oligosaccharide and polysaccharide characterization. The method developed employs ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC/QqQ-MS) analysis performed in multiple reaction monitoring (MRM) mode. A library of 22 glycosidic linkages was built using commercial oligosaccharide standards. Permethylation and hydrolysis conditions along with LC-MS/MS parameters were optimized resulting in a workflow requiring only 50 μg of substrate for the analysis. Samples were homogenized, permethylated, hydrolyzed, and then derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP) prior to analysis by UHPLC/MRM-MS. Separation by C18 reversed-phase UHPLC along with the simultaneous monitoring of derivatized terminal, linear, bisecting, and trisecting monosaccharide linkages by mass spectrometry is achieved within a 15 min run time. Reproducibility, efficacy, and robustness of the method was demonstrated with galactan ( Lupin) and polysaccharides within food such as whole carrots. The speed and specificity of the method enables its application toward the rapid glycosidic linkage analysis of oligosaccharides and polysaccharides.
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