ArticleeLife2018
Ancient mechanisms for the evolution of the bicoid homeodomain's function in fly development.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Mechanisms of Phenotypic Evolution from Molecules to Organisms: Integrating Evolutionary Biochemistry and Evolutionary Developmental Biology.Annual review of ecology, evolution, and systematics · 2026Article
- Asymmetric chromatin accessibility underlies anterior-posterior axis specification in moth fly embryos.PLoS biology · 2026Article
- From candidate genes to omics: Unbiased approaches reshaping arthropod Evo-Devo.Genetics and molecular biology · 2026Article
- Missense variants in human forkhead transcription factors reveal determinants of forkhead DNA bispecificity.Cell reports · 2025Article
- Genomic resources for the scuttle fly Megaselia abdita: a model organism for comparative developmental studies in flies.Development (Cambridge, England) · 2025Article
- Breaking anterior-posterior symmetry in the moth flybioRxiv : the preprint server for biology · 2025Article
- Versatile roles of disordered transcription factor effector domains in transcriptional regulation.The FEBS journal · 2025Review
- Missense variants in human forkhead transcription factors reveal determinants of forkhead DNA bispecificity.bioRxiv : the preprint server for biology · 2025Article
- Article
- Rugged fitness landscapes minimize promiscuity in the evolution of transcriptional repressors.Cell systems · 2024Article
- Primate protein-ligand interfaces exhibit significant conservation and unveil human-specific evolutionary drivers.PLoS computational biology · 2023Article
- Twenty-seven ZAD-ZNF genes of Drosophila melanogaster are orthologous to the embryo polarity determining mosquito gene cucoid.PloS one · 2023Article
- Evolution and loss of ß-catenin and TCF-dependent axis specification in insects.Current opinion in insect science · 2022Review
- Temperature-dependent genetics of thermotolerance between yeast species.Frontiers in ecology and evolution · 2022Article
- Joint effects of genes underlying a temperature specialization tradeoff in yeast.PLoS genetics · 2021Article
- Population and comparative genetics of thermotolerance divergence between yeast species.G3 (Bethesda, Md.) · 2021Article
- Suboptimal Intermediates Underlie Evolution of the Bicoid Homeodomain.Molecular biology and evolution · 2021Article
- Unexpected mutual regulation underlies paralogue functional diversification and promotes epithelial tissue maturation in Tribolium.Communications biology · 2020Article
- Structural studies of geranylgeranylglyceryl phosphate synthase, a prenyltransferase found in thermophilic Euryarchaeota.Acta crystallographica. Section D, Structural biology · 2020Article
- Evolutionary Dynamics of the SKN-1 → MED → END-1,3 Regulatory Gene Cascade inG3 (Bethesda, Md.) · 2020Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
The ancient mechanisms that caused developmental gene regulatory networks to diversify among distantly related taxa are not well understood. Here we use ancestral protein reconstruction, biochemical experiments, and developmental assays of transgenic animals carrying reconstructed ancestral genes to investigate how the transcription factor Bicoid (Bcd) evolved its central role in anterior-posterior patterning in flies. We show that most of Bcd's derived functions are attributable to evolutionary changes within its homeodomain (HD) during a phylogenetic interval >140 million years ago. A single substitution from this period (Q50K) accounts almost entirely for the evolution of Bcd's derived DNA specificity in vitro. In transgenic embryos expressing the reconstructed ancestral HD, however, Q50K confers activation of only a few of Bcd's transcriptional targets and yields a very partial rescue of anterior development. Adding a second historical substitution (M54R) confers regulation of additional Bcd targets and further rescues anterior development. These results indicate that two epistatically interacting mutations played a major role in the evolution of Bcd's controlling regulatory role in early development. They also show how ancestral sequence reconstruction can be combined with in vivo characterization of transgenic animals to illuminate the historical mechanisms of developmental evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.