ArticleThe ISME journal2019
Genome-scale fitness profile of Caulobacter crescentus grown in natural freshwater.
Article in The ISME journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
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- An N4-likebioRxiv : the preprint server for biology · 2025Article
- ThebioRxiv : the preprint server for biology · 2025Article
- A CRISPR-SpCas9M-reporting system for efficient and rapid genome editing in Caulobacter crescentus.Nucleic acids research · 2025Article
- A co-conserved gene pair supportsJournal of bacteriology · 2025Article
- A co-conserved gene pair supportsbioRxiv : the preprint server for biology · 2025Article
- Regulation ofmicroPublication biology · 2025Article
- Discovering genetic modulators of the protein homeostasis system through multilevel analysis.PNAS nexus · 2025Article
- Selective benefit of the sucrose TonB-dependent receptor, SucA, inmicroPublication biology · 2025Article
- Mutualism reduces the severity of gene disruptions in predictable ways across microbial communities.The ISME journal · 2023Article
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- A cryptic transcription factor regulates Caulobacter adhesin development.PLoS genetics · 2022Article
- Decoding the link of microbiome niches with homologous sequences enables accurately targeted protein structure prediction.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The ChvG-ChvI and NtrY-NtrX Two-Component Systems Coordinately Regulate Growth of Caulobacter crescentus.Journal of bacteriology · 2021Article
- Engineering High-Yield Biopolymer Secretion Creates an Extracellular Protein Matrix for Living Materials.mSystems · 2021Article
- The Dynamics of the Bacterial Community of the Photobioreactor-Cultivated Green MicroalgaBiology · 2021Article
- Covert Cross-Feeding Revealed by Genome-Wide Analysis of Fitness Determinants in a Synthetic Bacterial Mutualism.Applied and environmental microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial genomes evolve in complex ecosystems and are best understood in this natural context, but replicating such conditions in the lab is challenging. We used transposon sequencing to define the fitness consequences of gene disruption in the bacterium Caulobacter crescentus grown in natural freshwater, compared with axenic growth in common laboratory media. Gene disruptions in amino-acid and nucleotide sugar biosynthesis pathways and in metabolic substrate transport machinery impaired fitness in both lake water and defined minimal medium relative to complex peptone broth. Fitness in lake water was enhanced by insertions in genes required for flagellum biosynthesis and reduced by insertions in genes involved in biosynthesis of the holdfast surface adhesin. We further uncovered numerous hypothetical and uncharacterized genes for which disruption impaired fitness in lake water, defined minimal medium, or both. At the genome scale, the fitness profile of mutants cultivated in lake water was more similar to that in complex peptone broth than in defined minimal medium. Microfiltration of lake water did not significantly affect the terminal cell density or the fitness profile of the transposon mutant pool, suggesting that Caulobacter does not strongly interact with other microbes in this ecosystem on the measured timescale. Fitness of select mutants with defects in cell surface biosynthesis and environmental sensing were significantly more variable across days in lake water than in defined medium, presumably owing to day-to-day heterogeneity in the lake environment. This study reveals genetic interactions between Caulobacter and a natural freshwater environment, and provides a new avenue to study gene function in complex ecosystems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.