ArticleScientific reports2018
Chromosome replication as a measure of bacterial growth rate during Escherichia coli infection in the mouse peritonitis model.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
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- The gut microbiota and its biogeography.Nature reviews. Microbiology · 2024Review
- RNA polymerase redistribution supports growth in E. coli strains with a minimal number of rRNA operons.Nucleic acids research · 2023Article
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- Methods to monitor bacterial growth and replicative rates at the single-cell level.FEMS microbiology reviews · 2022Review
- L-Form Switching in Escherichia coli as a Common β-Lactam Resistance Mechanism.Microbiology spectrum · 2022Article
- Uropathogenic Escherichia coli subverts mitochondrial metabolism to enable intracellular bacterial pathogenesis in urinary tract infection.Nature microbiology · 2022Article
- Gasdermin D protects againstFrontiers in immunology · 2022Article
- Targeted chromosomal Escherichia coli:dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness.PLoS genetics · 2021Article
- Antisense inhibition of the Escherichia coli NrdAB aerobic ribonucleotide reductase is bactericidal due to induction of DNA strand breaks.The Journal of antimicrobial chemotherapy · 2021Article
- Bioinformatic prediction reveals posttranscriptional regulation of the chromosomal replication initiator geneRNA biology · 2021Article
- Replication Dynamics for Six Gram-Negative Bacterial Species during Bloodstream Infection.mBio · 2021Article
- Effect of erythritol and xylitol on Streptococcus pyogenes causing peritonsillar abscesses.Scientific reports · 2021Article
- A multifaceted cellular damage repair and prevention pathway promotes high-level tolerance to β-lactam antibiotics.EMBO reports · 2021Article
- A data-based mathematical modelling study to quantify the effects of ciprofloxacin and ampicillin on the within-host dynamics ofJournal of the Royal Society, Interface · 2020Article
- Counting Replication Origins to Measure Growth of Pathogens.Antibiotics (Basel, Switzerland) · 2020Article
- Bacteria under antibiotic attack: Different strategies for evolutionary adaptation.PLoS pathogens · 2020Review
- Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator.Nature communications · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The efficacy of most antibiotics is dependent on active bacterial growth, yet little is known about the growth dynamics during infection. Therefore, means to measure in-host bacterial growth rate is of importance. Here, we use chromosome replication as readout for in situ bacterial growth rate during infection; obtained from a single biological specimen. We have applied two independent methods: quantitative PCR (qPCR) and fluorescence microscopy, to quantify the level of chromosome replication present during Escherichia coli propagation in the mouse peritonitis model. We find that the methods complement each other and allow for quantification of growth rate, both on a population average and on a single-cell level. We demonstrate the presence of heterogeneous growth rates within bacterial populations propagating during infection. Also, no growth cessation was observed during the apparent stationary phase in vivo, and, by comparison of growth dynamics at different anatomical sites, we demonstrate that E. coli is unlikely to grow independently intravascularly. These findings provide novel insight into bacterial growth during host infection, and underscore the importance of pinpointing the primary site of infection in septicaemia of unknown origin and ensuring antibiotic availability at this site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.