Evidence map›Paper›PMID 30297374›Full record

ArticleDevelopment (Cambridge, England)2018

The Ric-8A/Gα13/FAK signalling cascade controls focal adhesion formation during neural crest cell migration in

Gabriela Toro-Tapia, Soraya Villaseca, Andrea Beyer, Alice Roycroft, Sylvain Marcellini, Roberto Mayor, Marcela Torrejón

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. GαFrontiers in cell and developmental biology · 2022
    Review
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Gabriela Toro-TapiaDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla 160-C, Concepción 4030000, Chile.
Soraya VillasecaDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla 160-C, Concepción 4030000, Chile.
Andrea BeyerDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla 160-C, Concepción 4030000, Chile.
Alice RoycroftDepartment of Cell and Developmental Biology, University College London, London WC1E 6BT, UK.
Sylvain MarcelliniDepartamento de Biología Cellular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla 160-C, Concepción 4030000, Chile.
Roberto MayorDepartment of Cell and Developmental Biology, University College London, London WC1E 6BT, UK matorrejon@udec.cl r.mayor@ucl.ac.uk.
Marcela TorrejónDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla 160-C, Concepción 4030000, Chile matorrejon@udec.cl r.mayor@ucl.ac.uk.ORCID 0000-0003-3393-9690
University of Concepción · CLUniversity College London · GB

Funding

Biotechnology and Biological Sciences Research Council M008517Medical Research Council J000655Medical Research Council M010465Wellcome Trust
6 · The paper itself

Abstract

Ric-8A is a pleiotropic guanine nucleotide exchange factor involved in the activation of various heterotrimeric G-protein pathways during adulthood and early development. Here, we sought to determine the downstream effectors of Ric-8A during the migration of the vertebrate cranial neural crest (NC) cells. We show that the Gα13 knockdown phenocopies the Ric-8A morphant condition, causing actin cytoskeleton alteration, protrusion instability, and a strong reduction in the number and dynamics of focal adhesions. In addition, the overexpression of Gα13 is sufficient to rescue Ric-8A-depleted cells. Ric-8A and Gα13 physically interact and colocalize in protrusions of the cells leading edge. The focal adhesion kinase FAK colocalizes and interacts with the endogenous Gα13, and a constitutively active form of Src efficiently rescues the Gα13 morphant phenotype in NC cells. We propose that Ric-8A-mediated Gα13 signalling is required for proper cranial NC cell migration by regulating focal adhesion dynamics and protrusion formation.

Indexed as

Cell MovementSignal TransductionActin CytoskeletonAnimalsCell AdhesionCell MembraneDown-RegulationEmbryo, NonmammalianFocal Adhesion Protein-Tyrosine KinasesFocal AdhesionsGTP-Binding Protein alpha Subunits, G12-G13Guanine Nucleotide Exchange FactorsModels, BiologicalMorpholinosNeural CrestPhenotypeFocal Adhesion Protein-Tyrosine KinasesGTP-Binding Protein alpha Subunits, G12-G13Guanine Nucleotide Exchange FactorsMorpholinosRic-8 protein, Xenopussrc-Family KinasesXenopus ProteinsCell migrationFocal adhesionGEFHeterotrimeric G-ProteinNeural crestProtrusion formationRic-8A

Identifiers

PMID30297374
PMCPMC6262797
OpenAlexW2896023018

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.