Evidence map›Paper›PMID 30296492›Full record

ArticleJournal of structural biology2018

Using Scipion for stream image processing at Cryo-EM facilities.

J Gómez-Blanco, J M de la Rosa-Trevín, R Marabini, L Del Cano, A Jiménez, M Martínez, R Melero, T Majtner, D Maluenda, J Mota and 15 more

Abstract read
In one paragraph

Article in Journal of structural biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Automated model building and protein identification in cryo-EM maps.bioRxiv : the preprint server for biology · 2023
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Journal of applied crystallography · 2022
    Article
  12. Article
  13. Article
  14. Article
  15. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  16. Single-Particle Cryo-EM of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  17. CryoEM structure ofLife science alliance · 2020
    Article
  18. Industrial cryo-EM facility setup and management.Acta crystallographica. Section D, Structural biology · 2020
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

J Gómez-BlancoDepartment of Anatomy and Cell Biology, McGill University, Montreal, Canada.
J M de la Rosa-TrevínDepartment of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
R MarabiniEscuela Politécnica Superior, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain. Electronic address: roberto.marabini@uam.es.
L Del CanoBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
A JiménezBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
M MartínezBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
R MeleroBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
T MajtnerBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
D MaluendaBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
J MotaBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
Y RancelBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
E Ramírez-AportelaBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
J L VilasBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
M CarroniDepartment of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
S FleischmannDepartment of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
E LindahlDepartment of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Stockholm, Sweden; Swedish e-Science Research Center, KTH Royal Institute of Technology, Stockholm, Sweden.
A W AshtonDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
M BashamDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
D K ClareDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
K SavageDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
C A SiebertDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
G G SharovMedical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 OQH, United Kingdom.
C O S SorzanoBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
P ConesaBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
J M CarazoBiocomputing Unit, National Center for Biotechnology (CSIC), C/ Darwin, 3, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.

Funding

Medical Research Council MC_UP_A025_1011Wellcome Trust 210711/Z/18/Z
6 · The paper itself

Abstract

Three dimensional electron microscopy is becoming a very data-intensive field in which vast amounts of experimental images are acquired at high speed. To manage such large-scale projects, we had previously developed a modular workflow system called Scipion (de la Rosa-Trevín et al., 2016). We present here a major extension of Scipion that allows processing of EM images while the data is being acquired. This approach helps to detect problems at early stages, saves computing time and provides users with a detailed evaluation of the data quality before the acquisition is finished. At present, Scipion has been deployed and is in production mode in seven Cryo-EM facilities throughout the world.

Indexed as

SoftwareAlgorithmsComputational BiologyCryoelectron MicroscopyImage Processing, Computer-AssistedImaging, Three-DimensionalReproducibility of ResultsElectron microscopyHigh throughputImage processingLive processingScipionStreaming

Identifiers

PMID30296492
PMCPMC6303188

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.