Evidence map›Paper›PMID 30295787›Full record

ReviewMicroscopy (Oxford, England)2019

Structured illumination approaches for super-resolution in plant cells.

Sidney L Shaw, David Thoms, James Powers

Abstract readReview
In one paragraph

Review in Microscopy (Oxford, England), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Review
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  4. Article
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  8. Review
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  10. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sidney L ShawDepartment of Biology, Light Microscopy Imaging Center, Indiana University, Bloomington, IN, USA.
David ThomsDepartment of Biology, Light Microscopy Imaging Center, Indiana University, Bloomington, IN, USA.
James PowersDepartment of Biology, Light Microscopy Imaging Center, Indiana University, Bloomington, IN, USA.

Funding

Acquisition of a DeltaVision OMX-SR Imaging SystemS10OD024988 · OD · INDIANA UNIVERSITY INDIANAPOLIS · PI WALCZAK, CLAIRE E · 2018 to 2018
$600k
NIH HHS S10 OD024988
6 · The paper itself

Abstract

The advent of super-resolution techniques in biological microscopy has opened new frontiers for exploring the molecular distribution of proteins and small molecules in cells. Improvements in optical design and innovations in the approaches for the collection of fluorescence emission have produced substantial gains in signal from chemical labels and fluorescent proteins. Structuring the illumination to elicit fluorescence from specific or even random patterns allows the extraction of higher order spatial frequencies from specimens labeled with conventional probes. Application of this approach to plant systems for super-resolution imaging has been relatively slow owing in large part to aberrations incurred when imaging through the plant cell wall. In this brief review, we address the use of two prominent methods for generating super-resolution images in living plant specimens and discuss future directions for gaining better access to these techniques.

Indexed as

ArabidopsisImaging, Three-DimensionalMicroscopy, FluorescenceOptical ImagingPlant CellsplantSIMstructured illumination

Identifiers

PMID30295787
PMCPMC6676951

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.