ArticleThe Journal of antimicrobial chemotherapy2019
WGS analysis of a penicillin-resistant Neisseria meningitidis strain containing a chromosomal ROB-1 β-lactamase gene.
Article in The Journal of antimicrobial chemotherapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Emergence ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2026Article
- Progression of antibiotic resistance inClinical microbiology reviews · 2025Review
- Geographical and temporal variations of serogroups and clonal types ofJournal of medical microbiology · 2025Article
- Investigation of invasiveFrontiers in public health · 2025Article
- Genetic characterization ofFrontiers in cellular and infection microbiology · 2024Article
- Evolution and exchange of plasmids in pathogenicmSphere · 2023Article
- Recent Evolution of Susceptibility to Beta-Lactams inAntibiotics (Basel, Switzerland) · 2023Article
- Antibiotic Susceptibility Profile for the USOpen forum infectious diseases · 2023Article
- Meningococcal disease in North America: Updates from the Global Meningococcal Initiative.The Journal of infection · 2022Review
- Demographic Features of Invasive Meningococcal Disease in Taiwan, 1993 to 2020, and Genetic Characteristics of Neisseria meningitidis Isolates, 2003 to 2020.Microbiology spectrum · 2022Article
- Article
- Serogroup Y Clonal Complex 23 Meningococcus in China Acquiring Penicillin Resistance from Commensal Neisseria lactamica Species.Antimicrobial agents and chemotherapy · 2022Article
- Epidemiology of invasive meningococcal disease in Canada, 2012-2019.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2022Article
- Surveillance and control of meningococcal disease in the COVID-19 era: A Global Meningococcal Initiative review.The Journal of infection · 2022Review
- Characterisation of Neisseria meningitidis cc11/ET-15 variant by whole genome sequencing.Memorias do Instituto Oswaldo Cruz · 2022Article
- Acquisition of Ciprofloxacin Resistance Among an Expanding Clade of β-Lactamase-Positive, Serogroup Y Neisseria meningitidis in the United States.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Detection of Ciprofloxacin-Resistant, β-Lactamase-Producing Neisseria meningitidis Serogroup Y Isolates - United States, 2019-2020.MMWR. Morbidity and mortality weekly report · 2020Article
- Article
- The spread of chloramphenicol-resistant Neisseria meningitidis in Southeast Asia.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Article
- Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Objectives: Neisseria meningitidis is rarely penicillin resistant. We describe WGS analysis of a penicillin-resistant N. meningitidis collected from a case of invasive meningococcal disease. Methods: Serogrouping, serotyping and serosubtyping were performed with specific antibodies. β-Lactamase was detected by nitrocefin. MICs were determined by Etest and agar dilution. Sequencing of N. meningitidis genomes was done on the Illumina MiSeq platform and genome data were analysed using the Bacterial Isolate Genome Sequence Database (BIGSdb) on the PubMLST Neisseria website (https://pubmlst.org/neisseria/). Transformation was used to confirm the genetic basis of the penicillin resistance. Results: An N. meningitidis blood isolate from a female patient in her mid-50s with a painful and septic left shoulder was found to have penicillin MIC values of 3-12 mg/L. The isolate was typed as Y: 14, 19: P1.- and ST3587, and was weakly β-lactamase positive. WGS analysis identified a full-length copy of the β-lactamase gene blaROB-1, which was contained on a 1719 bp insert with a G + C content of 41.7% (versus a G + C content of N. meningitidis of 51.7%), suggesting that the blaROB-1 gene came from a different bacterial species. A GenBank analysis of the blaROB-1 gene insert found 99.77% identity with a DNA segment found in plasmid pB1000' from Haemophilus influenzae. Transformation of a penicillin-susceptible strain with the blaROB-1 gene conferred β-lactamase activity and penicillin resistance. Conclusions: N. meningitidis serogroup Y, ST3587 can carry and express the blaROB-1 gene, leading to penicillin resistance. It is highly likely that the N. meningitidis isolate acquired the blaROB-1 gene from H. influenzae.
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