ArticleThe New phytologist2019
Towards take-all control: a C-21β oxidase required for acylation of triterpene defence compounds in oat.
Article in The New phytologist, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Biosynthesis and functions of triterpenoids in cereals.Journal of advanced research · 2025Review
- Comprehensive mutant chemotyping reveals embedding of a lineage-specific biosynthetic gene cluster in wider plant metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Roles of three cytochrome P450 monooxygenases in triterpene biosynthesis and their potential impact on growth and development.Plant physiology · 2024Article
- Creating saponin-free yellow pea seeds byPlant direct · 2024Article
- Characterization of the horse chestnut genome reveals the evolution of aescin and aesculin biosynthesis.Nature communications · 2023Article
- Selection for seed size has uneven effects on specialized metabolite abundance in oat (Avena sativa L.).G3 (Bethesda, Md.) · 2022Article
- Genome-Wide Association Mapping of Crown and Brown Rust Resistance in Perennial Ryegrass.Genes · 2021Article
- Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.Nature communications · 2021Article
- Non-target Site Herbicide Resistance Is Conferred by Two Distinct Mechanisms in Black-Grass (Frontiers in plant science · 2021Article
- Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology.Frontiers in plant science · 2021Review
- Plant terpenes that mediate below-ground interactions: prospects for bioengineering terpenoids for plant protection.Pest management science · 2019Review
- Molecular Basis of C-30 Product Regioselectivity of Legume Oxidases Involved in High-Value Triterpenoid Biosynthesis.Frontiers in plant science · 2019Article
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Authors and funding
10 authors.
Funding
Abstract
Oats produce avenacins, antifungal triterpenes that are synthesized in the roots and provide protection against take-all and other soilborne diseases. Avenacins are acylated at the carbon-21 position of the triterpene scaffold, a modification critical for antifungal activity. We have previously characterized several steps in the avenacin pathway, including those required for acylation. However, transfer of the acyl group to the scaffold requires the C-21β position to be oxidized first, by an as yet uncharacterized enzyme. We mined oat transcriptome data to identify candidate cytochrome P450 enzymes that may catalyse C-21β oxidation. Candidates were screened for activity by transient expression in Nicotiana benthamiana. We identified a cytochrome P450 enzyme AsCYP72A475 as a triterpene C-21β hydroxylase, and showed that expression of this enzyme together with early pathway steps yields C-21β oxidized avenacin intermediates. We further demonstrate that AsCYP72A475 is synonymous with Sad6, a previously uncharacterized locus required for avenacin biosynthesis. sad6 mutants are compromised in avenacin acylation and have enhanced disease susceptibility. The discovery of AsCYP72A475 represents an important advance in the understanding of triterpene biosynthesis and paves the way for engineering the avenacin pathway into wheat and other cereals for control of take-all and other diseases.
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