ArticleFrontiers in cellular neuroscience2018
Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy.
Article in Frontiers in cellular neuroscience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Optimizing fluorescence imaging through scattering media using structured light-assisted wavefront shaping.iScience · 2025Article
- Zebrafish fluorescence imaging platform based on Bessel light sheet illumination.Biomedical optics express · 2025Article
- Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity.Communications biology · 2024Article
- Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish.International journal of molecular sciences · 2023Review
- Multimodal Characterization of Seizures in Zebrafish Larvae.Biomedicines · 2022Article
- KHz-rate volumetric voltage imaging of the whole Zebrafish heart.Biophysical reports · 2022Article
- Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy.Biomedical optics express · 2022Article
- Label-free image-encoded microfluidic cell sorter with a scanning Bessel beam.APL photonics · 2021Article
- Reconstruction scheme for excitatory and inhibitory dynamics with quenched disorder: application to zebrafish imaging.Journal of computational neuroscience · 2021Article
- Colored visual stimuli evoke spectrally tuned neuronal responses across the central nervous system of zebrafish larvae.BMC biology · 2020Article
- Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy.Biomedical optics express · 2020Article
- Fast multi-directional DSLM for confocal detection without striping artifacts.Biomedical optics express · 2020Article
- Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes.Frontiers in neuroscience · 2020Review
- Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector.Journal of biomedical optics · 2019Article
- Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy.Neurophotonics · 2019Article
- Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.Frontiers in neuroanatomy · 2019Article
- Tailored Sample Mounting for Light-Sheet Fluorescence Microscopy of Clarified Specimens by Polydimethylsiloxane Casting.Frontiers in neuroanatomy · 2019Article
- Flexible Multi-Beam Light-Sheet Fluorescence Microscope for Live Imaging Without Striping Artifacts.Frontiers in neuroanatomy · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a method of choice to observe brain function with high frame rates at cellular resolution. Inherently to LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest and, during functional imaging, modulate fluorescence variations related to neuronal activity. Here, we report how Bessel beams reduce streaking artifacts and produce high-fidelity quantitative data demonstrating a fivefold increase in sensitivity to calcium transients and a 20-fold increase in accuracy in the detection of activity correlations in functional imaging. Furthermore, using principal component analysis, we show that measurements obtained with Bessel beams are clean enough to reveal in one-shot experiments correlations that can not be averaged over trials after stimuli as is the case when studying spontaneous activity. Our results not only demonstrate the contamination of data by systematic and random errors through conventional Gaussian illumination and but,furthermore, quantify the increase in fidelity of such data when using Bessel beams.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.