ArticleJournal of neuroscience methods2019
Quantitative and qualitative assessment of glymphatic flux using Evans blue albumin.
Article in Journal of neuroscience methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Albumin-binding dendrimer-conjugated siRNA enables safe and effective gene silencing throughout the central nervous system.Nucleic acids research · 2026Article
- The glymphatic system in Huntington's disease.Journal of Huntington's disease · 2025Review
- Time of Day Influences Concentrations of Total Protein and Albumin in Cerebrospinal Fluid in HIV.International journal of molecular sciences · 2023Article
- Fluid dynamics in aging-related dementias.Neurobiology of disease · 2023Review
- Article
- Perivascular Spaces, Glymphatic System and MR.Frontiers in neurology · 2022Review
- Quantitative Determination of Glymphatic Flow Using Spectrophotofluorometry.Neuroscience bulletin · 2020Article
- Pituitary Adenylate Cyclase-Activating Polypeptide Attenuates Brain Edema by Protecting Blood-Brain Barrier and Glymphatic System After Subarachnoid Hemorrhage in Rats.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2020Article
- Impaired neural differentiation and glymphatic CSF flow in theDisease models & mechanisms · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe glymphatic system is a proposed pathway for clearance of proteins and macromolecules from brain, and disrupted glymphatic flux is implicated in neurological disease. We capitalized on colorimetric, fluorescent, and protein-binding properties of Evans blue to evaluate glymphatic flux. NEW
methodTwenty-five μL of 1% Evans blue-labeled albumin (EBA) in artificial cerebrospinal fluid (aCSF) was injected into the intracisternal space of anesthetized postnatal day 17 rats. Serum was collected at various time points after injection (n = 37) and EBA was measured spectrophotometrically. In separate rats (n = 3), a cranial window was placed over the parietal cortex and EBA transit was evaluated using in vivo multiphoton microscopy. Separate rats (n = 6) were processed for immunohistochemistry to examine localization of EBA. In some rats, intracranial pressure (ICP) was increased via intracisternal injection of aCSF.
resultsEBA was detected in serum as early as 30 min, was maximal at 4 h, and was undetectable at 72 h after intracisternal injection. Using intra-vital microscopy and immunohistochemistry EBA could be tracked from CSF to perivascular locations. Consistent with removal via glymphatic flux, increasing ICP to 40 mmHg accelerated transit of EBA from CSF to blood. COMPARISON WITH EXISTING
methodsTransit of EBA from CSF to serum could be quantified spectrophotometrically without radioactive labeling. Glymphatic flux could also be qualitatively evaluated using EBA fluorescence.
conclusionWe present a novel technique for simultaneous quantitative and qualitative evaluation of glymphatic flux in rats.
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Registered trials
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