ArticleIUBMB life2018
Massive mitochondrial DNA content in diplonemid and kinetoplastid protists.
Article in IUBMB life, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
- Preparation of Diplonemid Samples for Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing.BMC genomics · 2025Article
- Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa).RNA (New York, N.Y.) · 2025Article
- Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi.Communications biology · 2025Article
- Article
- The genome sequences of the diplonemid protistWellcome open research · 2025Article
- Probing mechanical selection in diverse eukaryotic genomes through accurate prediction of 3D DNA mechanics.bioRxiv : the preprint server for biology · 2024Article
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Miniature RNAs are embedded in an exceptionally protein-rich mitoribosome via an elaborate assembly pathway.Nucleic acids research · 2023Article
- Recent expansion of metabolic versatility in Diplonema papillatum, the model species of a highly speciose group of marine eukaryotes.BMC biology · 2023Article
- Hydrophobicity-Driven Increases in Editing in Mitochondrial mRNAs during the Evolution of Kinetoplastids.Molecular biology and evolution · 2023Article
- Novel insertions in the mitochondrial maxicircle ofParasitology · 2022Article
- Detection ofPathogens (Basel, Switzerland) · 2022Article
- Typical structure of rRNA coding genes in diplonemids points to two independent origins of the bizarre rDNA structures of euglenozoans.BMC ecology and evolution · 2022Article
- Trophic flexibility of marine diplonemids - switching from osmotrophy to bacterivory.The ISME journal · 2022Article
- Delineating transitions during the evolution of specialised peroxisomes: Glycosome formation in kinetoplastid and diplonemid protists.Frontiers in cell and developmental biology · 2022Article
- Mitochondrial RNA editing inComputational and structural biotechnology journal · 2022Article
- Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum.BMC biology · 2021Article
- Vestiges of the Bacterial Signal Recognition Particle-Based Protein Targeting in Mitochondria.Molecular biology and evolution · 2021Article
- Molecular Diagnosis of Leishmaniasis: Quantification of Parasite Load by a Real-Time PCR Assay with High Sensitivity.Pathogens (Basel, Switzerland) · 2021Article
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
The mitochondrial DNA of diplonemid and kinetoplastid protists is known for its suite of bizarre features, including the presence of concatenated circular molecules, extensive trans-splicing and various forms of RNA editing. Here we report on the existence of another remarkable characteristic: hyper-inflated DNA content. We estimated the total amount of mitochondrial DNA in four kinetoplastid species (Trypanosoma brucei, Trypanoplasma borreli, Cryptobia helicis, and Perkinsela sp.) and the diplonemid Diplonema papillatum. Staining with 4',6-diamidino-2-phenylindole and RedDot1 followed by color deconvolution and quantification revealed massive inflation in the total amount of DNA in their organelles. This was further confirmed by electron microscopy. The most extreme case is the ∼260 Mbp of DNA in the mitochondrion of Diplonema, which greatly exceeds that in its nucleus; this is, to our knowledge, the largest amount of DNA described in any organelle. Perkinsela sp. has a total mitochondrial DNA content ~6.6× greater than its nuclear genome. This mass of DNA occupies most of the volume of the Perkinsela cell, despite the fact that it contains only six protein-coding genes. Why so much DNA? We propose that these bloated mitochondrial DNAs accumulated by a ratchet-like process. Despite their excessive nature, the synthesis and maintenance of these mtDNAs must incur a relatively low cost, considering that diplonemids are one of the most ubiquitous and speciose protist groups in the ocean. © 2018 IUBMB Life, 70(12):1267-1274, 2018.
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