Evidence map›Paper›PMID 30290760›Full record

ArticleBMC urology2018

Detection of micro-metastases by flow cytometry in lymph nodes from patients with penile cancer.

Lu Zhang, Jin Hu, A Ali Zirakzadeh, Jesper Rosvall, Mats Hedlund, Ping Sheng Hu, Robert P A Wallin, Amir Sherif, Ola Winqvist

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC urology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 80% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Lu ZhangDepartment of Medicine, Immunology and Allergy Unit, Karolinska University Hospital, SE-171 76, Stockholm, Sweden.
Jin HuDepartment of Medicine, Immunology and Allergy Unit, Karolinska University Hospital, SE-171 76, Stockholm, Sweden.
A Ali ZirakzadehDepartment of Medicine, Immunology and Allergy Unit, Karolinska University Hospital, SE-171 76, Stockholm, Sweden.
Jesper RosvallDepartment of Urology, South General Hospital, Karolinska Institutet, Stockholm, Stockholm, Sweden.
Mats HedlundDepartment of Urology, South General Hospital, Karolinska Institutet, Stockholm, Stockholm, Sweden.
Ping Sheng HuCancer Biotherapy Center, Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Robert P A WallinDepartment of Medicine, Immunology and Allergy Unit, Karolinska University Hospital, SE-171 76, Stockholm, Sweden.
Amir SherifDepartment of Surgical and Perioperative Sciences, Urology and Andrology, Umeå University, Umeå, Sweden.
Ola WinqvistDepartment of Medicine, Immunology and Allergy Unit, Karolinska University Hospital, SE-171 76, Stockholm, Sweden. ola.winqvist@sll.se.
Karolinska University Hospital · SEKarolinska Institutet · SEGuiyang Medical University · CNUmeå University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe tumor draining lymph node concept was first described in penile cancer for staging. Immunohistochemistry and histopathology evaluations are routinely used in clinical practice to examine lymph nodes for metastasis. However, these methods are time-consuming with low diagnostic accuracy and micro-metastases might be missed. In this study, we aim to evaluate detection of metastatic cells in draining lymph nodes by flow cytometry.

methodsTo assess the sensitivity of micro-metastasis detection by FACS (Fluorescence-activated cell sorting), HeLa cells were titrated into Peripheral blood mononuclear cells (PBMCs) and expression of pan-cytokeratin AE1/AE3 was analyzed. Single cell suspensions were separately prepared from 10 regional lymph nodes obtained from 5 patients with invasive penile cancer undergoing radical surgery and lymph node dissection. Lymph node dereived cells were examined for cell surface expression of EpCAM, E-cadherin and intracellular expression of pan-cytokeratin AE1/AE3 by FACS.

resultsTen lymph nodes from 5 penile cancer patients were investigated in a head-to-head comparison between FACS and pathology examination of sections. All metastatic lymph nodes verified by pathology examination were also identified by FACS. Two additional lymph nodes with micro-metastases were diagnosed by FACS only.

conclusionsFACS analyses of pan-cytokeratin AE1/AE3 stained single cells from tumor draining lymph nodes can be used to detect micro-metastases in patients with penile cancer patients.

Indexed as

Flow CytometryAgedAged, 80 and overAntigens, CDBiomarkersCadherinsEpithelial Cell Adhesion MoleculeHeLa CellsHumansKeratinsLeukocytes, MononuclearLymphatic MetastasisLymph NodesMaleNeoplasm MicrometastasisPenile NeoplasmsAntigens, CDBiomarkersCadherinsCDH1 protein, humanEPCAM protein, humanEpithelial Cell Adhesion MoleculeKeratinsFlow cytometryMicro-metastasis detectionPan-cytokeratin AE1/AE3Penile cancerTumor draining lymph nodes

Identifiers

PMID30290760
PMCPMC6173905
OpenAlexW2894777766

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.