Evidence map›Paper›PMID 30288485›Full record

ArticleInternational journal of reproductive biomedicine2018

Evaluation of specific germ cell genes expression in mouse embryonic stem cell-derived germ cell like cells treated with bone morphogenetic protein 4 in vitro.

Maryam Gholamitabar Tabari, Seyed Gholam Ali Jorsaraei, Mohammad Ghasemzadeh-Hasankolaei, Ali Asghar Ahmadi, Neda Mahdinezhad Gorji

Open access · goldAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of reproductive biomedicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Maryam Gholamitabar TabariInfertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.
Seyed Gholam Ali JorsaraeiInfertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.
Mohammad Ghasemzadeh-HasankolaeiInfertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.
Ali Asghar AhmadiInfertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.
Neda Mahdinezhad GorjiInfertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.
Babol University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBone morphogenetic protein 4 (BMP4) is a significant signaling molecule that involves in initiating of differentiation and performs multifunctional effects on embryonic stem cells (ESCs) and embryos.

objectiveThe goal of the present study was to evaluate an in vitro differentiation model of mouse embryonic stem cells into germ cells, using BMP4. MATERIALS AND

methodsin this experimental study, we used Oct4-GFP mouse ESCs to form embryoid body (EB) aggregations for two days. Then, single cells from EB were cultured for four days with BMP4. Using MTT assay and gene expression levels for evaluation of Mvh and Riken by real-time RT-PCR of six concentrations, 12.5 ng/ ml BMP4 was determined as an optimized dose. Then, the expression level of

resultsIn the +BMP4 group, the genes encoding Riken (p≤0.001) and

conclusionDown-regulation of

Indexed as

Bone morphogenetic proteinEmbryoid bodyEmbryonic stem cellsGerm cells

Identifiers

PMID30288485
PMCPMC6163050
OpenAlexW2892983625

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.