Evidence map›Paper›PMID 30288138›Full record

ArticleMaterials today (Kidlington, England)2018

Microfluidic generation of transient cell volume exchange for convectively driven intracellular delivery of large macromolecules.

Anna Liu, Muhymin Islam, Nicholas Stone, Vikram Varadarajan, Jenny Jeong, Sam Bowie, Peng Qiu, Edmund K Waller, Alexander Alexeev, Todd Sulchek

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Materials today (Kidlington, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

  1. Article
  2. Exploiting Device Deformability for Fluid and Particle Manipulation.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Review
  3. Article
  4. Article
  5. The Rise of Mechanobiology for Advanced Cell Engineering and Manufacturing.Advanced materials (Deerfield Beach, Fla.) · 2025
    Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Anna LiuDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Muhymin IslamSchool of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Nicholas StoneSchool of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Vikram VaradarajanDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Jenny JeongDepartment of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Sam BowieSchool of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Peng QiuDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Edmund K WallerDepartment of Hematology and Medical Oncology, Emory University School of Medicine, Atlanta, Georgia, USA.
Alexander AlexeevSchool of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Todd SulchekDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.

Funding

Label-free microfluidic enrichment of cancer cells from noncancer cells in ascites fluidR21CA191243 · NCI · GEORGIA INSTITUTE OF TECHNOLOGY · PI MCDONALD, JOHN F, SULCHEK, TODD · 2016 to 2019
$593k
Microfluidic Monitoring of Single Cell Elasticity, Viscoelasticity, and PlasticityR21EB020977 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI ALEXEEV, ALEXANDER, LAM, WILBUR A · 2015 to 2016
$405k
NCI NIH HHS R21 CA191243NIBIB NIH HHS R21 EB020977
6 · The paper itself

Abstract

Efficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering and other biomedical applications. We discovered a unique cell biophysical phenomenon of transient cell volume exchange by using microfluidics to rapidly and repeatedly compress cells. This behavior consists of brief, mechanically induced cell volume loss followed by rapid volume recovery. We harness this behavior for high-throughput, convective intracellular delivery of large polysaccharides (2000 kDa), particles (100 nm), and plasmids while maintaining high cell viability. Successful proof of concept experiments in transfection and intracellular labeling demonstrated potential to overcome the most prohibitive challenges in intracellular delivery for cell engineering.

Indexed as

Cell deformationCell engineeringDeliveryMicrofluidics

Identifiers

PMID30288138
PMCPMC6166476

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.