ArticleScientific reports2018
Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Review
- Validating COI eDNA Metabarcoding Primers for Detection of Subterranean Fauna.Molecular ecology resources · 2026Article
- Dietary DNA Metabarcoding From Animal Fecal Samples.Current protocols · 2026Article
- Article
- Expanding Monitoring Capacity for Potential Invasive Species in Arctic Canada With Environmental DNA Metabarcoding.Global change biology · 2025Article
- A long-term ecological research dataset from the marine genetic monitoring programme ARMS-MBON 2020-2021.Biodiversity data journal · 2025Article
- Article
- Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes.FEMS microbiology reviews · 2024Review
- A belly full of jelly? DNA metabarcoding shows evidence for gelatinous zooplankton predation by several fish species in Greenland waters.Royal Society open science · 2024Article
- Mare-MAGE curated reference database of fish mitochondrial genes.BMC genomic data · 2023Article
- Article
- Environmental RNA as a Tool for Marine Community Biodiversity Assessments.Scientific reports · 2022Article
- Monitoring of benthic eukaryotic communities in two tropical coastal lagoons through eDNA metabarcoding: a spatial and temporal approximation.Scientific reports · 2022Article
- Article
- Hidden diversity of Ctenophora revealed by new mitochondrial COI primers and sequences.Molecular ecology resources · 2022Article
- High-throughput sequencing outperforms traditional morphological methods in Blue Catfish diet analysis and reveals novel insights into diet ecology.Ecology and evolution · 2021Article
- eDNA metabarcoding of small plankton samples to detect fish larvae and their preys from Atlantic and Pacific waters.Scientific reports · 2021Article
- A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region.Royal Society open science · 2021Article
- Article
- The crossover from microscopy to genes in marine diversity: from species to assemblages in marine pelagic copepods.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We establish the new approach of environmental DNA (eDNA) analyses for the North Sea. Our study uses a multigene approach, including the mitochondrial cytochrome-c-oxidase subunit I (COI) gene for analyzing species composition and the nuclear hypervariable region V8 of 18S rDNA for analyzing supraspecific biodiversity. A new minibarcode primer (124 bp) was created on the basis of a metazoan COI barcode library with 506 species and tested in silico, in vitro, and in situ. We applied high throughput sequencing to filtrates of 23 near-bottom water samples taken at three seasons from 14 stations. The set of COI primers allowed amplification of mitochondrial minibarcodes for diverse metazoan phyla and the differentiation at the species level for more than 99% of the specimens in the dataset. Our results revealed that the number of sequences is not consistent with proportions in the given DNA mixture. Altogether, environmental sequences could be assigned to 114 species and to 12 metazoan phyla. A spatial distribution of taxa recovered by eDNA was congruent with known distributions. Finally, the successful detection of species and biodiversity depends on a comprehensive sequence reference database. Our study offers a powerful tool for future biodiversity research, including the detection of nonnative species.
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