ArticleScientific reports2018
Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- GRASPing experience-dependent protein expression signatures enriched for hippocampal engram cell synapses.Science advances · 2026Article
- Translational Remodeling of the Synaptic Proteome During Aging.Aging cell · 2025Article
- mRNA and circRNA mislocalization to synapses are key features of Alzheimer's disease.PLoS genetics · 2024Article
- MAPK signaling and a mobile scaffold complex regulate AMPA receptor transport to modulate synaptic strength.Cell reports · 2022Article
- Surface charge manipulation and electrostatic immobilization of synaptosomes for super-resolution imaging: a study on tau compartmentalization.Scientific reports · 2021Article
- A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines.Nature neuroscience · 2021Article
- Quantitative Synaptic Biology: A Perspective on Techniques, Numbers and Expectations.International journal of molecular sciences · 2020Review
- A comparative analysis of the mobility of 45 proteins in the synaptic bouton.The EMBO journal · 2020Article
- Local translation in neurons: visualization and function.Nature structural & molecular biology · 2019Review
- Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.Scientific reports · 2019Article
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6 authors.
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Abstract
Protein copy numbers can be measured by biochemical methods ranging from quantitative Western Blotting to several mass spectrometry approaches. Such methods only provide average copy numbers, obtained over large cell numbers. However, copy number estimates for single cells or single organelles could be obtained by combining biochemical characterizations with an imaging approach. We performed this here for synaptic proteins, in a protocol that we termed comparative synaptosome imaging for semi-quantitative copy numbers (CosiQuant). In brief, in CosiQuant we immunostain in parallel biochemically-characterized synaptosomes, for which we have already determined the average protein copy numbers, and the samples of interest (such as neuronal cultures). We then derive the copy numbers in the samples of interest by comparing the immunofluorescence intensities. We measured the intensities not only in arbitrary fluorescence units, but also as numbers of antibodies per synaptosome, for a large number of targets. This implies that other groups can immediately apply CosiQuant for these targets, by simply estimating the number of antibodies per structure of interest. CosiQuant should therefore be a useful addition to the growing set of imaging techniques for synaptic neuroscience.
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