Evidence map›Paper›PMID 30287546›Full record

ArticleMolecular & cellular proteomics : MCP2019

Quantitative Proteomics Identification of Seminal Fluid Proteins in Male

Irem Sepil, Ben R Hopkins, Rebecca Dean, Marie-Laëtitia Thézénas, Philip D Charles, Rebecca Konietzny, Roman Fischer, Benedikt M Kessler, Stuart Wigby

Open access · hybridAbstract read
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 100 citations in OpenAlex.

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  18. The life history ofProceedings of the National Academy of Sciences of the United States of America · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Irem SepilFrom the ‡Edward Grey Institute, Department of Zoology, University of Oxford, Oxford, UK;. Electronic address: irem.sepil@zoo.ox.ac.uk.
Ben R HopkinsFrom the ‡Edward Grey Institute, Department of Zoology, University of Oxford, Oxford, UK.
Rebecca DeanDepartment of Genetics, Evolution and Environment, University College London, London, UK.
Marie-Laëtitia ThézénasTDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Philip D CharlesTDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Rebecca KonietznyTDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Roman FischerTDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Benedikt M KesslerTDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Stuart WigbyFrom the ‡Edward Grey Institute, Department of Zoology, University of Oxford, Oxford, UK.
University of Oxford · GBUniversity College London · GB

Funding

Biotechnology and Biological Sciences Research Council BB/K014544/1
6 · The paper itself

Abstract

Seminal fluid contains some of the fastest evolving proteins currently known. These seminal fluid proteins (Sfps) play crucial roles in reproduction, such as supporting sperm function, and particularly in insects, modifying female physiology and behavior. Identification of Sfps in small animals is challenging, and often relies on samples taken from the female reproductive tract after mating. A key pitfall of this method is that it might miss Sfps that are of low abundance because of dilution in the female-derived sample or rapid processing in females. Here we present a new and complementary method, which provides added sensitivity to Sfp identification. We applied label-free quantitative proteomics to

Indexed as

AnimalsAnimal StructuresDatabases, ProteinDrosophila melanogasterDrosophila ProteinsFemaleGenitalia, FemaleMaleProteomeProteomicsReproductionSeminal Plasma ProteinsDrosophila ProteinsProteomeSeminal Plasma ProteinsAnimal models*Drosophila melanogaster*Label-free quantificationProtein Identification*Tandem Mass Spectrometry

Identifiers

PMID30287546
PMCPMC6427238
OpenAlexW2952552869

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.