Evidence map›Paper›PMID 30287259›Full record

ArticleClinica chimica acta; international journal of clinical chemistry2018

An ELISA for quantifying GPIHBP1 autoantibodies and making a diagnosis of the GPIHBP1 autoantibody syndrome.

Kazuya Miyashita, Isamu Fukamachi, Tetsuo Machida, Kiyomi Nakajima, Stephen G Young, Masami Murakami, Anne P Beigneux, Katsuyuki Nakajima

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinica chimica acta; international journal of clinical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Chylomicronemia from GPIHBP1 autoantibodies.Journal of lipid research · 2020
    Review
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Kazuya MiyashitaImmuno Biological Laboratories, Fujioka, Gunma, Japan.
Isamu FukamachiImmuno Biological Laboratories, Fujioka, Gunma, Japan.
Tetsuo MachidaDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Kiyomi NakajimaDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Stephen G YoungDepartment of Medicine and David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90025, United States; Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90025, United States.
Masami MurakamiDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Anne P BeigneuxDepartment of Medicine and David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90025, United States. Electronic address: abeigneux@mednet.ucla.edu.
Katsuyuki NakajimaDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan. Electronic address: nakajimak05@ybb.ne.jp.
Gunma University · JPUniversity of California, Los Angeles · USMedical & Biological Laboratories (Japan) · JP

Funding

The Lipin Protein Family and Triglyceride Metabolism P01HL090553 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YOUNG, STEPHEN G. · 2008 to 2018
$23.3M
Understanding the Influence of Lipid Homeostasis on T cell FunctionP01HL146358 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TONTONOZ, PETER J · 2019 to 2023
$11.7M
Refining Physiologic Mechanisms for Intravascular Triglyceride MetabolismR01HL087228 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BEIGNEUX, ANNE, FONG, LOREN GI · 2007 to 2024
$7.8M
Investigating Mechanisms for Lipid Transport in Health and DiseaseR35HL139725 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YOUNG, STEPHEN G. · 2018 to 2024
$6.1M
New Avenues in GPIHBP1 ResearchR01HL094732 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BEIGNEUX, ANNE · 2009 to 2012
$1.8M
NHLBI NIH HHS P01 HL090553NHLBI NIH HHS P01 HL146358NHLBI NIH HHS R01 HL087228NHLBI NIH HHS R01 HL094732NHLBI NIH HHS R35 HL139725
6 · The paper itself

Abstract

backgroundAutoantibodies against GPIHBP1, the endothelial cell transporter for lipoprotein lipase (LPL), cause severe hypertriglyceridemia ("GPIHBP1 autoantibody syndrome"). Affected patients have low serum GPIHBP1 and LPL levels. We report the development of a sensitive and specific ELISA, suitable for routine clinical use, to detect GPIHBP1 autoantibodies in serum and plasma.

methodsSerum and plasma samples were added to wells of an ELISA plate that had been coated with recombinant human GPIHBP1. GPIHBP1 autoantibodies bound to GPIHBP1 were detected with an HRP-labeled antibody against human immunoglobulin. Sensitivity, specificity, and reproducibility of the ELISA was evaluated with plasma or serum samples from patients with the GPIHBP1 autoantibody syndrome.

resultsA solid-phase ELISA to detect and quantify GPIHBP1 autoantibodies in human plasma and serum was developed. Spiking recombinant human GPIHBP1 into the samples reduced the ability of the ELISA to detect GPIHBP1 autoantibodies. The ELISA is reproducible and sensitive; it can detect GPIHBP1 autoantibodies in samples diluted by >1000-fold.

conclusionWe have developed a sensitive and specific ELISA for detecting GPIHBP1 autoantibodies in human serum and plasma; this assay will make it possible to rapidly diagnose the GPIHBP1 autoantibody syndrome.

Indexed as

Enzyme-Linked Immunosorbent AssayAdultAutoantibodiesFemaleHumansMaleMiddle AgedReceptors, LipoproteinSyndromeYoung AdultAutoantibodiesGPIHBP1 protein, humanReceptors, LipoproteinAutoimmune diseaseELISAGPIHBP1 autoantibodiesLipolysisLipoprotein lipaseLPL

Identifiers

PMID30287259
PMCPMC6349362
OpenAlexW2895758289

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.