Evidence map›Paper›PMID 30287125›Full record

ArticleThe Lancet. Global health2018

Use of quantitative molecular diagnostic methods to investigate the effect of enteropathogen infections on linear growth in children in low-resource settings: longitudinal analysis of results from the MAL-ED cohort study.

Elizabeth T Rogawski, Jie Liu, James A Platts-Mills, Furqan Kabir, Paphavee Lertsethtakarn, Mery Siguas, Shaila S Khan, Ira Praharaj, Arinao Murei, Rosemary Nshama and 40 more

Open access · goldAbstract read
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In one paragraph

Article in The Lancet. Global health, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 261 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
261citing papers in PubMed, 4 pooled it
22.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

261 citing papers in PubMed, 4 syntheses or guidelines pooled it, 384 citations in OpenAlex.

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  4. Consequences of Shigella infection in young children: a systematic review.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2023
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  20. Analysis ofInfection and immunity · 2026
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201 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

50 authors at 17 institutions in 10 countries.

Elizabeth T RogawskiDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA; Department of Public Health Sciences, University of Virginia, Charlottesville, VA, USA.
Jie LiuDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA.
James A Platts-MillsDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA.
Furqan KabirAga Khan University, Karachi, Pakistan.
Paphavee LertsethtakarnArmed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand.
Mery SiguasAsociación Benéfica PRISMA, Iquitos, Peru.
Shaila S KhanInternational Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Ira PraharajChristian Medical College, Vellore, India.
Arinao MureiUniversity of Venda, Thohoyandou, South Africa.
Rosemary NshamaHaydom Global Health Institute, Haydom, Tanzania.
Buliga MujagaKilimanjaro Clinical Research Institute, Moshi, Tanzania.
Alexandre HavtFederal University of Ceara, Fortaleza, Brazil.
Irene A MacielFundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
Darwin J OperarioDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA.
Mami TaniuchiDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA.
Jean GratzDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA.
Suzanne E StroupDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA.
James H RobertsDepartment of Public Health Sciences, University of Virginia, Charlottesville, VA, USA.
Adil KalamAga Khan University, Karachi, Pakistan.
Fatima AzizAga Khan University, Karachi, Pakistan.
Shahida QureshiAga Khan University, Karachi, Pakistan.
M Ohedul IslamInternational Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Pimmada SakpaisalArmed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand.
Sasikorn SilapongArmed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand.
Pablo P YoriAsociación Benéfica PRISMA, Iquitos, Peru; Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Revathi RajendiranChristian Medical College, Vellore, India.
Blossom BennyChristian Medical College, Vellore, India.
Monica McGrathBloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA; Fogarty International Center, National Institutes of Health, Bethesda, MD, USA.
Jessica C SeidmanFogarty International Center, National Institutes of Health, Bethesda, MD, USA.
Dennis LangFoundation for the National Institutes of Health, Bethesda, MD, USA.
Michael GottliebFoundation for the National Institutes of Health, Bethesda, MD, USA.
Richard L GuerrantDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA.
Aldo A M LimaFederal University of Ceara, Fortaleza, Brazil.
Jose Paulo LeiteFundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
Amidou SamieUniversity of Venda, Thohoyandou, South Africa.
Pascal O BessongUniversity of Venda, Thohoyandou, South Africa.
Nicola PageNational Institute for Communicable Diseases, Johannesburg, South Africa.
Ladaporn BodhidattaFogarty International Center, National Institutes of Health, Bethesda, MD, USA.
Carl MasonArmed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand.
Sanjaya ShresthaWalter Reed/AFRIMS Research Unit, Nepal, Kathmandu, Nepal; University of Bergen, Bergen, Norway.
Ireen KiweluKilimanjaro Clinical Research Institute, Moshi, Tanzania.
Estomih R MdumaHaydom Global Health Institute, Haydom, Tanzania.
Najeeha T IqbalAga Khan University, Karachi, Pakistan.
Zulfiqar A BhuttaAga Khan University, Karachi, Pakistan.
Tahmeed AhmedInternational Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Rashidul HaqueInternational Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Gagandeep KangChristian Medical College, Vellore, India.
Margaret N KosekAsociación Benéfica PRISMA, Iquitos, Peru; Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Eric R HouptDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA. Electronic address: erh6k@hscmail.mcc.virginia.edu.
MAL-ED Network Investigators
University of Virginia · USAga Khan University · PKInternational Centre for Diarrhoeal Disease Research · BDArmed Forces Research Institute of Medical Science · THChristian Medical College & Hospital · INFundação Oswaldo Cruz · BRUniversidade Federal do Ceará · BRUniversity of Bergen · NOUniversity of Venda · ZAHaydom Lutheran Hospital · TZJohns Hopkins University · USFogarty International Center · USFoundation for the National Institutes of Health · USKilimanjaro Christian Medical Centre · TZNational Institute for Communicable DiseasesPrisma · PEUniversity of Virginia Health System · US

Funding

Quantitative molecular diagnostics for diarrhea etiology and vaccine efficacyK23AI114888 · NIAID · UNIVERSITY OF VIRGINIA · PI PLATTS-MILLS, JAMES ALEXANDER · 2015 to 2019
$932k
Translation of epidemiologic findings in enteric disease to public health actionK01AI130326 · NIAID · UNIVERSITY OF VIRGINIA · PI ROGAWSKI MCQUADE, ELIZABETH TACKET · 2018 to 2022
$612k
NIAID NIH HHS K01 AI130326NIAID NIH HHS K23 AI114888
6 · The paper itself

Abstract

backgroundEnteropathogen infections in early childhood not only cause diarrhoea but contribute to poor growth. We used molecular diagnostics to assess whether particular enteropathogens were associated with linear growth across seven low-resource settings.

methodsWe used quantitative PCR to detect 29 enteropathogens in diarrhoeal and non-diarrhoeal stools collected from children in the first 2 years of life obtained during the Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development (MAL-ED) multisite cohort study. Length was measured monthly. We estimated associations between aetiology-specific diarrhoea and subclinical enteropathogen infection and quantity and attained length in 3 month intervals, at age 2 and 5 years, and used a longitudinal model to account for temporality and time-dependent confounding.

findingsAmong 1469 children who completed 2 year follow-up, 35 622 stool samples were tested and yielded valid results. Diarrhoeal episodes attributed to bacteria and parasites, but not viruses, were associated with small decreases in length after 3 months and at age 2 years. Substantial decrements in length at 2 years were associated with subclinical, non-diarrhoeal, infection with Shigella (length-for-age Z score [LAZ] reduction -0·14, 95% CI -0·27 to -0·01), enteroaggregative Escherichia coli (-0·21, -0·37 to -0·05), Campylobacter (-0·17, -0·32 to -0·01), and Giardia (-0·17, -0·30 to -0·05). Norovirus, Cryptosporidium, typical enteropathogenic E coli, and Enterocytozoon bieneusi were also associated with small decrements in LAZ. Shigella and E bieneusi were associated with the largest decreases in LAZ per log increase in quantity per g of stool (-0·13 LAZ, 95% CI -0·22 to -0·03 for Shigella; -0·14, -0·26 to -0·02 for E bieneusi). Based on these models, interventions that successfully decrease exposure to Shigella, enteroaggregative E coli, Campylobacter, and Giardia could increase mean length of children by 0·12-0·37 LAZ (0·4-1·2 cm) at the MAL-ED sites.

interpretationSubclinical infection and quantity of pathogens, particularly Shigella, enteroaggregative E coli, Campylobacter, and Giardia, had a substantial negative association with linear growth, which was sustained during the first 2 years of life, and in some cases, to 5 years. Successfully reducing exposure to certain pathogens might reduce global stunting.

fundingBill & Melinda Gates Foundation.

Indexed as

Asia, WesternBrazilChild, PreschoolCohort StudiesDiarrheaEnterobacteriaceae InfectionsGrowth DisordersHealth ResourcesHumansInfantInfant, NewbornMolecular Diagnostic TechniquesPeruReal-Time Polymerase Chain ReactionSouth AfricaTanzania

Identifiers

PMID30287125
PMCPMC6227248
OpenAlexW2893167551

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.