ArticleStem cell research & therapy2018
A robust cell culture system for large scale feeder cell-free expansion of human breast epithelial progenitors.
Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1.NPJ Regenerative medicine · 2025Article
- The Mayo Clinic Salivary Tissue-Organoid Biobanking: A Resource for Salivary Regeneration Research.bioRxiv : the preprint server for biology · 2024Article
- Towards organoid culture without Matrigel.Communications biology · 2021Review
- Organoids as ComplexCellular and molecular bioengineering · 2020Review
- Isolation and characterization of a new basal-like luminal progenitor in human breast tissue.Stem cell research & therapy · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNormal human breast epithelial cells are maintained by the proliferation and differentiation of different human breast epithelial progenitors (HBEPs). However, these progenitor subsets can only be obtained at low frequencies, limiting their further characterization. Recently, it was reported that HBEPs can be minimally expanded in Matrigel cocultures with stromal feeder cells. However, variability of generating healthy feeder cells significantly impacts the effective expansion of HBEPs.
methodsHere, we report a robust feeder cell-free culture system for large-scale expansion of HBEPs in two-dimensional cultures.
resultsUsing this cell culture system HBEPs can be exponentially expanded as bulk cultures. Moreover, purified HBEP subtypes can also be separately expanded using our cell culture system. The expanded HBEPs retain their undifferentiated phenotype and form distinct epithelial colonies in colony forming cell assays.
conclusionsThe availability of a culture system enabling the large-scale expansion of HBEPs facilitates their application to screening platforms and other cell-based assays.
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