ArticlePloS one2018
Rapid identification of Bactrocera zonata (Dip.: Tephritidae) using TaqMan real-time PCR assay.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Simple, rapid and cost-effective DNA extraction techniques for detection of economically important fruit flies in India.MethodsX · 2026Article
- Article
- Mitochondrial genome of Bactrocera fruit flies (Tephritidae: Dacini): features, structure, and significance for diagnosis.BMC genomics · 2025Article
- Rapid visual detection assay for Bactrocera dorsalis (Hendel) using recombinase polymerase amplification and CRISPR/Cas12b.Scientific reports · 2025Article
- Diagnostic Tool for the Identification ofInsects · 2024Article
- A simple PCR-based quick detection of the economically important oriental fruit fly,Frontiers in plant science · 2024Article
- Rapid and sensitive on-site genetic diagnostics of pest fruit flies using CRISPR-Cas12a.Pest management science · 2023Article
- Loop-Mediated Isothermal Amplification (LAMP) and SYBR Green qPCR for Fast and Reliable Detection ofPlants (Basel, Switzerland) · 2022Article
- Rapid Detection ofPlants (Basel, Switzerland) · 2021Article
- Development of a loop-mediated isothermal amplification (LAMP) assay for the identification of the invasive wood borer3 Biotech · 2021Article
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Authors and funding
5 authors.
Funding
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Abstract
Tephritid fruit flies are ranked as one of the most damaging groups of insect pests. Morphological identification of fruit flies is mainly performed on adults due to the lack of adequate identification keys for immature stages. The peach fruit fly, Bactrocera zonata (Saunders), infests some of the principal commercial fruits and vegetables. It is, therefore important to avert its global dispersal, particularly by accurately identifying this species at ports of entry. In this study, a TaqMan real-time polymerase chain reaction (PCR) was developed for the accurate identification and sensitive detection of the peach fruit fly. A novel set of primers and probe were designed to specifically identify the mitochondrial cytochrome oxidase I (COI) gene. All specimens of peach fruit fly (including various life stages) were detected, and no cross reactivity with other tested tephritids were observed. Since this assay performed equally well with crushed insects and purified DNA, we note added efficiency by eliminating DNA extraction step. Considering the speed, specificity as well as sensitivity of the assay, Taqman real-time PCR can be used as a swift and specific method for pest species at ports of entry.
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