ArticlePloS one2018
An improved method for extraction of polar and charged metabolites from cyanobacteria.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 41 citations in OpenAlex.
- Phytochemical diversity of the metabolite profiles relates to the biostimulatory potential of agriculturally beneficial cyanobacteria.Archives of microbiology · 2025Article
- Comparative metabolites profiling of different solvent extracts of Asparagus species cladodes using liquid chromatography-mass spectrometry-based metabolomics and molecular networking.Phytochemical analysis : PCA · 2025Article
- Intracellular metabolomic profiling of Picochlorum sp. under diurnal conditions mimicking outdoor light, temperature, and seasonal variations.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Disrupted HPlant molecular biology · 2024Article
- Metabolite profiling of plant growth promoting cyanobacteria-3 Biotech · 2024Article
- Metabolomics and modelling approaches for systems metabolic engineering.Metabolic engineering communications · 2022Review
- Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas.RSC advances · 2022Review
- Review
- Probing Light-Dependent Regulation of the Calvin Cycle Using a Multi-Omics Approach.Frontiers in plant science · 2021Article
- Photosynthetic Co-Production of Succinate and Ethylene in A Fast-Growing Cyanobacterium,Metabolites · 2020Article
- Evaluation of freely available software tools for untargeted quantification ofMetabolic engineering communications · 2020Article
- A Novel Cyanobacterium Synechococcus elongatus PCC 11802 has Distinct Genomic and Metabolomic Characteristics Compared to its Neighbor PCC 11801.Scientific reports · 2020Article
- Metabolic engineering of a fast-growing cyanobacteriumBiotechnology for biofuels · 2020Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A key requirement for 13C Metabolic flux analysis (13C-MFA), a widely used technique to estimate intracellular metabolic fluxes, is an efficient method for the extraction of intermediate metabolites for analysis via liquid chromatography mass spectrometry (LC/MS). The 13C isotopic labeling results in further distribution of an already sparse pool of intermediate metabolites into isotopologues, each appearing as a separate chromatographic feature. We examined some of the reported solvent systems for the extraction of polar intracellular metabolites from three strains of cyanobacteria of the genus Synechococcus, viz., Synechococcus sp. PCC 7002, Synechococcus elongatus PCC 7942, and a newly isolated Synechococcus elongatus PCC 11801 (manuscript under review). High resolution-LC/MS was used to assess the relative abundance of the extracted metabolites. The different solvent systems used for extraction led to statistically significant changes in the extraction efficiency for a large number of metabolites. While a few hundred m/z features or potential metabolites were detected with different solvent systems, the abundance of over a quarter of all metabolites varied significantly from one solvent system to another. Further, the extraction methods were evaluated for a targeted set of metabolites that are important in 13C-MFA studies of photosynthetic organisms. While for the strain PCC 7002, the reported method using methanol-chloroform-water system gave satisfactory results, a mild base in the form of NH4OH had to be used in place of water to achieve adequate levels of extraction for PCC 7942 and PCC 11801. While minor changes in extraction solvent resulted in dramatic changes in the extraction efficiency of a number of compounds, certain metabolites such as amino acids and organic acids were adequately extracted in all the solvent systems tested. Overall, we present a new improved method for extraction using a methanol-chloroform-NH4OH system. Our method improves the extraction of polar compounds such as sugar phosphates, bisphosphates, that are central to 13C-MFA studies.
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