ArticleBioTechniques2018
The use of commercially available adhesive tapes to preserve cartilage and bone tissue integrity during cryosectioning.
Article in BioTechniques, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 12 citations in OpenAlex.
- A scale CUBIC-based clearing protocol preserves fluorescence in mouse rib bone and cartilage.JBMR plus · 2026Article
- A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.Histochemistry and cell biology · 2024Article
- Histological assessment of microtia cartilage, a potential source of autograft tissue in ear reconstruction.Journal of anatomy · 2024Article
- Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications.Bone research · 2024Review
- Alleviation of arthritis through prevention of neutrophil extracellular traps by an orally available inhibitor of protein arginine deiminase 4.Scientific reports · 2023Article
- A murine model of large-scale bone regeneration reveals a selective requirement for Sonic Hedgehog.NPJ Regenerative medicine · 2022Article
- ER Stress in ERp57 Knockout Knee Joint Chondrocytes Induces Osteoarthritic Cartilage Degradation and Osteophyte Formation.International journal of molecular sciences · 2021Article
- Methodology, selection, and integration of fracture healing assessments in mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Review
- SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism.Scientific reports · 2021Article
- Immunostaining of Skeletal Tissues.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A novel cryo-embedding method for in-depth analysis of craniofacial mini pig bone specimens.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The use of fluorescent tags to monitor protein expression and to lineage-trace cells has become a standard complement to standard histological techniques in the fields of embryology, pathology and regenerative medicine. Unfortunately, traditional paraffin embedding protocols can substantially diminish or abolish the native emission signal of the fluorophore of interest. To preserve the fluorescent signal, an alternative is to use cryosectioning; however, this can often result in undesirable artefacts such as tearing or shattering - particularly for mineralized tissues such as bone and cartilage. Here we present a method of using a commercially available tape to stabilize murine femur tissue, thus allowing for cryosectioning of cartilage and bone tissues carrying fluorescent tags without the need for demineralization.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.