ArticleEnvironmental science & technology2018
Screening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters.
Article in Environmental science & technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 93 citations in OpenAlex.
- Microbial synthesis and degradation of bio-based biodegradable plastics.Nature reviews. Microbiology · 2026Review
- Polyesterase activity and thermostability of carboxylesterases from Thermoleophilum album YS-3.The FEBS journal · 2026Article
- Application of the YebF secretion pathway inRSC chemical biology · 2026Article
- Cold-adapted carboxylesterases from Alcanivoracaceae active with a wide range of synthetic polyesters.Applied microbiology and biotechnology · 2026Article
- Structure-guided engineering of a gatekeeper residue enhances polylactic acid depolymerization by a cutinase fromFrontiers in bioengineering and biotechnology · 2026Article
- Plastic leachates alter the composition of marine microbial communities, not functional potential for plastic degradation.FEMS microbiology ecology · 2025Article
- Functional Enrichment and Sequence-Based Discovery Identify Promiscuous and Efficient Poly Lactic Acid Degrading Enzymes.Environmental science & technology · 2025Article
- Enzyme-Embedded Biodegradable Plastic for Sustainable Applications: Advances, Challenges, and Perspectives.ACS applied bio materials · 2025Review
- PEZy-miner: An artificial intelligence driven approach for the discovery of plastic-degrading enzyme candidates.Metabolic engineering communications · 2024Article
- Plastic-Degrading Enzymes from Marine Microorganisms and Their Potential Value in Recycling Technologies.Marine drugs · 2024Review
- Eco-friendly approaches for mitigating plastic pollution: advancements and implications for a greener future.Biodegradation · 2024Review
- Enzymatic degradation of polylactic acid (PLA).Applied microbiology and biotechnology · 2024Review
- Surface-associated residues in subtilisins contribute to poly-L-lactic acid depolymerization via enzyme adsorption.Microbial biotechnology · 2024Article
- Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization.Nature communications · 2024Article
- Biodegradation of Typical Plastics: From Microbial Diversity to Metabolic Mechanisms.International journal of molecular sciences · 2024Review
- Searching for new plastic-degrading enzymes from the plastisphere of alpine soils using a metagenomic mining approach.PloS one · 2024Article
- Microorganisms that produce enzymes active on biodegradable polyesters are ubiquitous.Biodegradation · 2023Review
- Design and construction of microbial cell factories based on systems biology.Synthetic and systems biotechnology · 2023Review
- Thermophilic Carboxylesterases from Hydrothermal Vents of the Volcanic Island of Ischia Active on Synthetic and Biobased Polymers and Mycotoxins.Applied and environmental microbiology · 2023Article
- Biodegradation of Biodegradable Polymers in Mesophilic Aerobic Environments.International journal of molecular sciences · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
21 authors at 5 institutions in 5 countries.
Funding
Abstract
The continuous growth of global plastics production, including polyesters, has resulted in increasing plastic pollution and subsequent negative environmental impacts. Therefore, enzyme-catalyzed depolymerization of synthetic polyesters as a plastics recycling approach has become a focus of research. In this study, we screened over 200 purified uncharacterized hydrolases from environmental metagenomes and sequenced microbial genomes and identified at least 10 proteins with high hydrolytic activity against synthetic polyesters. These include the metagenomic esterases MGS0156 and GEN0105, which hydrolyzed polylactic acid (PLA), polycaprolactone, as well as bis(benzoyloxyethyl)-terephthalate. With solid PLA as a substrate, both enzymes produced a mixture of lactic acid monomers, dimers, and higher oligomers as products. The crystal structure of MGS0156 was determined at 1.95 Å resolution and revealed a modified α/β hydrolase fold, with a lid domain and highly hydrophobic active site. Mutational studies of MGS0156 identified the residues critical for hydrolytic activity against both polyester and monoester substrates, with two-times higher polyesterase activity in the MGS0156 L169A mutant protein. Thus, our work identified novel, highly active polyesterases in environmental metagenomes and provided molecular insights into their activity, thereby augmenting our understanding of enzymatic polyester hydrolysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.