ArticleExtremophiles : life under extreme conditions2019
High diversity of thermophilic cyanobacteria in Rupite hot spring identified by microscopy, cultivation, single-cell PCR and amplicon sequencing.
Article in Extremophiles : life under extreme conditions, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Fungi dominate eukaryotic microbial assemblages in hot spring biofilms of East and Southeast Asia.Environmental microbiome · 2025Article
- Morphological and Molecular Characterization of Cyanobacteria Isolated from Two Geothermal Springs of the Central Ecuadorian Andes.Microorganisms · 2025Article
- Genotypic and molecular characterization of a moderately thermophilic cyanobacterium,Engineering microbiology · 2025Article
- Superior Resolution Profiling of the Coleofasciculus Microbiome by Amplicon Sequencing of the Complete 16S rRNA Gene and ITS Region.Environmental microbiology reports · 2025Article
- Thermophilic bacteria employ a contractile injection system in hot spring microbial mats.The ISME journal · 2025Article
- Isolation, Identification and Pigment Analysis of Novel Cyanobacterial Strains from Thermal Springs.Plants (Basel, Switzerland) · 2024Article
- Molecular Screening for Cyanobacteria and Their Cyanotoxin Potential in Diverse Habitats.Toxins · 2024Article
- Article
- Thermophilic cyanobacteria-exciting, yet challenging biotechnological chassis.Applied microbiology and biotechnology · 2024Review
- Hyperthermophile diversity microbes in the Calientes geothermal field, Tacna, Peru.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs.Extremophiles : life under extreme conditions · 2023Review
- Loss of Biliverdin Reductase Increases Oxidative Stress in the CyanobacteriumMicroorganisms · 2023Article
- Comparative analysis of bacterial diversity in two hot springs in Hefei, China.Scientific reports · 2023Article
- Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis.Environmental microbiome · 2022Article
- The Green MicroalgaLife (Basel, Switzerland) · 2022Article
- Genome-Wide Investigation and Analysis of Microsatellites and Compound Microsatellites inLife (Basel, Switzerland) · 2021Article
- Bioactive Metabolites Produced by Cyanobacteria for Growth Adaptation and Their Pharmacological Properties.Biology · 2021Review
- Genomic and Phenotypic Characterization ofFrontiers in microbiology · 2021Article
- Description, Taxonomy, and Comparative Genomics of a Novel species,Frontiers in microbiology · 2021Article
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11 authors.
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Abstract
Genotypic and morphological diversity of cyanobacteria in the Rupite hot spring (Bulgaria) was investigated by means of optical microscopy, cultivation, single-cell PCR, and 16S rRNA gene amplicon sequencing. Altogether, 34 sites were investigated along the 71-39 °C temperature gradient. Analysis of samples from eight representative sites shown that Illumina, optical microscopy, and Roche 454 identified 72, 45 and 19% respective occurrences of all cumulatively present taxa. Optical microscopy failed to detect species of minor occurrence; whereas, amplicon sequencing technologies suffered from failed primer annealing and the presence of species with extensive extracellular polysaccharides production. Amplicon sequencing of the 16S rRNA gene V5-V6 region performed by Illumina identified the cyanobacteria most reliably to the generic level. Nevertheless, only the combined use of optical microscopy, cultivation and sequencing methods allowed for reliable estimate of the cyanobacterial diversity. Here, we show that Rupite hot-spring system hosts one of the richest cyanobacterial flora reported from a single site above 50 °C. Chlorogloeopsis sp. was the most abundant at the highest temperature (68 °C), followed by Leptolyngbya boryana, Thermoleptolyngbya albertanoae, Synechococcus bigranulatus, Oculatella sp., and Desertifilum sp. thriving above 60 °C, while Leptolyngbya geysericola, Geitlerinema splendidum, and Cyanobacterium aponinum were found above 50 °C.
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