Evidence map›Paper›PMID 30284103›Full record

ArticleJournal of assisted reproduction and genetics2019

Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development.

Sujith Raj Salian, Guruprasad Nayak, Sandhya Kumari, Sandesh Patel, Shruthi Gowda, Yashaswini Shenoy, Sinoy Sugunan, Rajanikant G K, Renuka Suresh Managuli, Srinivas Mutalik and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Sujith Raj SalianDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Guruprasad NayakDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Sandhya KumariDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Sandesh PatelDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Shruthi GowdaDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Yashaswini ShenoyDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Sinoy SugunanSchool of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala, 673601, India.
Rajanikant G KSchool of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala, 673601, India.
Renuka Suresh ManaguliDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Srinivas MutalikDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Vandana DahiyaDepartment of Chemistry, Indian Institute of Technology Jodhpur, Karwar, India.
Samanwita PalDepartment of Chemistry, Indian Institute of Technology Jodhpur, Karwar, India.
Satish Kumar AdigaDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Guruprasad KalthurDepartment of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India. guru.kalthur@manipal.edu.ORCID http://orcid.org/0000-0002-4554-2917
Manipal Academy of Higher Education · INIndian Institute of Technology Jodhpur · INNational Institute of Technology Calicut · IN

Funding

Indian council of medical research ICMR/5/10/10/2009-RHN
6 · The paper itself

Abstract

purposeMotility of spermatozoa helps not only in planning the type of infertility treatment but also directly reflects the success rate in assisted reproductive technology (ART). Previously, biotin, a water-soluble vitamin, has been shown to increase the motility and longevity of cryopreserved human spermatozoa. The present study was designed to understand the molecular basis of the beneficial effects of presence of biotin in sperm wash medium on early embryo development.

methodsThe effect biotin supplementation to sperm wash medium on the sperm parameters were assessed in swim-up fraction of normozoospermic and asthenozoospermic ejaculates collected from infertile men. Fertilization and early embryo development was studied using Swiss albino mice.

resultsEven though both biotin and pentoxifylline (PTX) enhanced the motility of spermatozoa from normozoospermic and asthenozoospermic samples, biotin group exhibited higher in vitro survival. Using mouse model, we observed that presence of biotin or PTX in sperm wash medium improved the fertilization rate and blastocyst rate compared to control. Blastocysts from these groups had significantly higher total cell number (P < 0.01) and lower apoptotic index. In silico target prediction revealed that GTPase HRas (HRas), tyrosine-protein phosphatase nonreceptor type 1 (PTP1B), and glucokinase are the probable targets for biotin. Solution-state Nuclear Magnetic Resonance (NMR) studies confirmed that biotin interacts both with human HRas and PTP1B.

conclusionOur results indicate that presence of biotin in sperm wash medium can improve the fertilization potential and preimplantation embryo development and can be considered as a safe alternate to PTX.

Indexed as

AnimalsAsthenozoospermiaBiotinBlastocystCryopreservationCulture MediaEmbryonic DevelopmentFemaleFertilizationFertilization in VitroGene Expression Regulation, DevelopmentalGlucokinaseHumansMaleMicePentoxifyllineBiotinCulture MediaGlucokinasePentoxifyllineProtein Tyrosine Phosphatase, Non-Receptor Type 1Ptpn1 protein, mouseBiotinBlastocyst rateFertilizationPentoxifyllineSperm motility

Identifiers

PMID30284103
PMCPMC6420554
OpenAlexW2895342017

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.