Evidence map›Paper›PMID 30283472›Full record

ArticleFrontiers in plant science2018

Light-Dependent Changes in the Spatial Localization of Metabolites in

Patrick A McVey, Liza E Alexander, Xinyu Fu, Bo Xie, Katherine-Jo Galayda, Basil J Nikolau, Robert S Houk

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Patrick A McVeyDepartment of Chemistry, Iowa State University, Ames, IA, United States.
Liza E AlexanderAmes Laboratory-US DOE, Ames, IA, United States.
Xinyu FuAmes Laboratory-US DOE, Ames, IA, United States.
Bo XieAmes Laboratory-US DOE, Ames, IA, United States.
Katherine-Jo GalaydaDepartment of Chemistry, Iowa State University, Ames, IA, United States.
Basil J NikolauAmes Laboratory-US DOE, Ames, IA, United States.
Robert S HoukDepartment of Chemistry, Iowa State University, Ames, IA, United States.
Iowa State University · USAmes National Laboratory · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. However, many non-colored compounds are also present; their cellular distributions are not readily identifiable optically. In this study we evaluate the applicability of mass spectrometry imaging (MSI) via electrospray laser desorption ionization (ELDI) to reveal the spatial distribution of metabolites. ELDI-MSI is a matrix free, atmospheric pressure ionization method that utilizes a UV laser coupled with supplemental ionization by electrospray. We specifically applied ELDI-MSI to determine the spatial distribution of metabolites in Coleus Henna half leaves that were grown with half-sections either fully illuminated or shaded. We monitored dynamic changes in the spatial distribution of metabolites in response to the change of illumination every 7 days for a 28 day period. A novel source-sink relationship was observed between the 2 halves of the experimental leaf. Furthermore, Coleus Henna leaves present visually recognizable sectors associated with the differential accumulation of flavonoids. Thus, we correlated the effect of differential illumination and presence or absence of flavonoids with metabolic changes revealed by the accumulation of carbohydrates, amino acids, and organic acids. The results show the potential of ELDI-MSI to provide spatial information for a variety of plant metabolites with little sample preparation.

Indexed as

anthocyaninColeus (Solenostemon scuttellarioides)electrospray laser desorption ionizationflavonoidmass spectrometry imagingmatrix-free imagingshadingsource-to-sink

Identifiers

PMID30283472
PMCPMC6156358
OpenAlexW2891426606

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.