ArticleScientific reports2018
moxMaple3: a Photoswitchable Fluorescent Protein for PALM and Protein Highlighting in Oxidizing Cellular Environments.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation.BMC research notes · 2025Article
- Single-molecule tracking of PprI inFrontiers in microbiology · 2023Article
- First biphotochromic fluorescent protein moxSAASoti stabilized for oxidizing environment.Scientific reports · 2022Article
- The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti.Scientific reports · 2021Article
- Gatekeeper function for Short stop at the ring canals of the Drosophila ovary.Current biology : CB · 2021Article
- Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy.Frontiers in molecular biosciences · 2021Article
- Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM.Molecular biology of the cell · 2019Article
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Authors and funding
4 authors.
Funding
Abstract
The ability of fluorescent proteins (FPs) to fold robustly is fundamental to the autocatalytic formation of the chromophore. While the importance of the tertiary protein structure is well appreciated, the impact of individual amino acid mutations for FPs is often not intuitive and requires direct testing. In this study, we describe the engineering of a monomeric photoswitchable FP, moxMaple3, for use in oxidizing cellular environments, especially the eukaryotic secretory pathway. Surprisingly, a point mutation to replace a cysteine substantially improved the yield of correctly folded FP capable of chromophore formation, regardless of cellular environment. The improved folding of moxMaple3 increases the fraction of visibly tagged fusion proteins, as well as FP performance in PALM super-resolution microscopy, and thus makes moxMaple3 a robust monomeric FP choice for PALM and optical highlighting applications.
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Registered trials
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