Evidence map›Paper›PMID 30282508›Full record

ArticleUpsala journal of medical sciences2018

Differences in secretome in culture media when comparing blastocysts and arrested embryos using multiplex proximity assay.

Karin E Lindgren, Fatma Gülen Yaldir, Julius Hreinsson, Jan Holte, Karin Kårehed, Inger Sundström-Poromaa, Helena Kaihola, Helena Åkerud

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Upsala journal of medical sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Karin E Lindgrena Department of Women's and Children's Health , Uppsala University , SE-751 85 Uppsala , Sweden.
Fatma Gülen Yaldira Department of Women's and Children's Health , Uppsala University , SE-751 85 Uppsala , Sweden.
Julius Hreinssonb Department of Immunology, Genetics and Pathology , Uppsala University , SE-751 85 Uppsala , Sweden.
Jan Holtea Department of Women's and Children's Health , Uppsala University , SE-751 85 Uppsala , Sweden.
Karin Kåreheda Department of Women's and Children's Health , Uppsala University , SE-751 85 Uppsala , Sweden.
Inger Sundström-Poromaaa Department of Women's and Children's Health , Uppsala University , SE-751 85 Uppsala , Sweden.
Helena Kaiholab Department of Immunology, Genetics and Pathology , Uppsala University , SE-751 85 Uppsala , Sweden.
Helena Åkerudb Department of Immunology, Genetics and Pathology , Uppsala University , SE-751 85 Uppsala , Sweden.
Uppsala University · SECarl von Linné Clinic · SEKarolinska Institutet · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe aim of this study was to assess different patterns of the human embryo secretome analysed as protein levels in culture media. Furthermore, analyses to correlate protein levels with quality and timing to development of human embryos were performed. MATERIAL AND

methodsHuman day-2 cryopreserved embryos were cultured for four days in an EmbryoScope

resultsSpecific patterns of timing of development of blastocysts were found, where a difference in time to start of cavitation was found between high- and low-quality blastocysts. There appeared to be a correlation between specific protein patterns and successful formation of morulae and blastocysts. Embryos developing into blastocysts had higher levels of EMMPRIN than arrested embryos, and levels of caspase-3 were lower in high- versus low-quality blastocysts. Also, higher levels of VEGF-A, IL-6, and EMMPRIN correlated with shorter times to morula formation.

conclusionsThe secretome and timing to development differ in embryos forming blastocysts and those that become arrested, and in high- versus low-quality blastocysts. The levels of certain proteins also correlate to specific times to development.

Indexed as

Gene Expression Regulation, DevelopmentalBasiginBlastocystCaspase 3CryopreservationCulture MediaDevelopmental BiologyEmbryo Culture TechniquesFertilization in VitroHumansInterleukin-6MorulaRetrospective StudiesSensitivity and SpecificityTime FactorsVascular Endothelial Growth Factor ABasiginBSG protein, humanCASP3 protein, humanCaspase 3Culture MediaIL6 protein, humanInterleukin-6Vascular Endothelial Growth Factor AVEGFA protein, humanBlastocystcaspase-3extracellular matrix metalloproteinase inducerinterleukin-6predictionsecretometime-lapsevascular endothelial growth factor A

Identifiers

PMID30282508
PMCPMC6198226
OpenAlexW2894732263

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.