Evidence map›Paper›PMID 30279530›Full record

ArticleScientific reports2018

Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite.

June-Sun Yoon, Dipak K Sahoo, Indu B Maiti, Subba R Palli

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
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  4. Review
  5. RNA Interference Past and Future Applications in Plants.International journal of molecular sciences · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

June-Sun YoonDepartment of Entomology, University of Kentucky, Lexington, Kentucky, 40546, USA.
Dipak K SahooKTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546, USA.
Indu B MaitiKTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546, USA.
Subba R PalliDepartment of Entomology, University of Kentucky, Lexington, Kentucky, 40546, USA. rpalli@email.uky.edu.ORCID http://orcid.org/0000-0002-0873-3247
University of Kentucky · USUniversity of Kentucky HealthCare · US

Funding

Molecular Analysis of Juvenile Hormone ActionR01GM070559 · NIGMS · UNIVERSITY OF KENTUCKY · PI PALLI, SUBBA R · 2005 to 2024
$4.1M
NIGMS NIH HHS R01 GM070559U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1R21AI131427-01
6 · The paper itself

Abstract

RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids [Methoprene-tolerant (Met), retinoid X receptor β, farnesoic acid O-methyltransferase, and CREB-binding protein] caused 35-56% mortality. Transgenic tobacco plants expressing hairpin dsRNA targeting Met gene were generated and tested. About 48% mortality was observed in TSSM raised on transgenic tobacco plants expressing dsMet. These studies not only broaden our knowledge on understanding hormone action in TSSM but also identified target genes that could be used in RNAi-mediated control of TSSM.

Indexed as

RNA InterferenceAnimalsArthropod ProteinsNicotianaPest Control, BiologicalPlants, Genetically ModifiedRNA, Small InterferingSurvival AnalysisTetranychidaeArthropod ProteinsRNA, Small Interfering

Identifiers

PMID30279530
PMCPMC6168543
OpenAlexW2894392372

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.