Evidence map›Paper›PMID 30278860›Full record

ArticleComparative medicine2018

Identification of Carboxylesterase, Butyrylcholinesterase, Acetylcholinesterase, Paraoxonase, and Albumin Pseudoesterase in Guinea Pig Plasma through Nondenaturing Gel Electrophoresis.

Geoffroy Napon, Alicia J Dafferner, Ashima Saxena, Oksana Lockridge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Comparative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 10 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Geoffroy NaponEppley Institute, University of Nebraska Medical Center, Omaha, Nebraska, USA; University of Nebraska-Omaha, Omaha, Nebraska, USA.
Alicia J DaffernerEppley Institute, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Ashima SaxenaDivision of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA; US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Oksana LockridgeEppley Institute, University of Nebraska Medical Center, Omaha, Nebraska, USA. olockrid@unmc.edu.
University of Nebraska Medical Center · USWalter Reed Army Institute of Research · US

Funding

UNMC/EPPLEY CANCER CENTER SUPPORT GRANTP30CA036727 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Joann B. Sweasy · 1985 to 2026
$55.0M
NCI NIH HHS P30 CA036727
6 · The paper itself

Abstract

Drugs to protect against nerve agent toxicity are tested in animals. The current preferred small animal model is guinea pigs because their plasma bioscavenging capacity resembles that of NHP. We stained nondenaturing polyacrylamide slab gels with a variety of substrates, inhibitors, and antibodies to identify the esterases in heparinized guinea pig plasma. An intense band of carboxylesterase activity migrated behind albumin. Minor carboxylesterase bands were revealed after background activity from paraoxonase was inhibited by using EDTA. The major butyrylcholinesterase band was a disulfide-linked dimer. Incubation with the antihuman butyrylcholinesterase antibody B2 18-5 shifted the butyrylcholinesterase dimer band to slower migrating complexes. Carboxylesterases were distinguished from butyrylcholinesterase by their sensitivity to inhibition by bis-

Indexed as

Guinea PigsAcetylcholinesteraseAlbuminsAnimalsAryldialkylphosphataseBlood Chemical AnalysisButyrylcholinesteraseCarboxylesteraseElectrophoresis, Polyacrylamide GelPlasmaRats, Sprague-DawleyAcetylcholinesteraseAlbuminsAryldialkylphosphataseButyrylcholinesteraseCarboxylesterase

Identifiers

PMID30278860
PMCPMC6200033
OpenAlexW2895485619

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.