ArticlePloS one2018
Properties of viable lyopreserved amnion are equivalent to viable cryopreserved amnion with the convenience of ambient storage.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Randomized Clinical Trial to Compare Cryopreserved and Lyopreserved Umbilical Cord Tissue to Treat Complex Diabetic Foot Wounds.The international journal of lower extremity wounds · 2026Trial
- Determinants of extraction efficiency in human amniotic membrane processing: biological, mechanical, and biochemical factors shaping growth factor yield.Cell and tissue banking · 2026Review
- A Multicenter, Randomized, Controlled, Clinical Trial Evaluating a Lyopreserved Amniotic Membrane in the Treatment of Venous Leg Ulcers.Health science reports · 2025Article
- Lyophilization of dehydrated human amniotic membrane: a proactive approach to preserve growth factors for enhanced wound healing.Cell and tissue banking · 2025Article
- An important detail that is still not clear in amniotic membrane applications: How do we store the amniotic membrane best?Cell and tissue banking · 2024Review
- Inter-placental variability is not a major factor affecting the healing efficiency of amniotic membrane when used for treating chronic non-healing wounds.Cell and tissue banking · 2023Article
- Connective tissue matrices from placental disc for wound healing: mini-review.Biotechnology letters · 2023Review
- Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane.Bioengineering (Basel, Switzerland) · 2023Article
- Hypothermic Preservation of Adipose-Derived Mesenchymal Stromal Cells as a Viable Solution for the Storage and Distribution of Cell Therapy Products.Bioengineering (Basel, Switzerland) · 2022Article
- Long-term and short-term preservation strategies for tissue engineering and regenerative medicine products: state of the art and emerging trends.PNAS nexus · 2022Review
- Standardized reporting of amnion and amnion/chorion allograft data for wound care.Health science reports · 2022Article
- The healing dynamics of non-healing wounds using cryo-preserved amniotic membrane.International wound journal · 2022Article
- Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing.Scientific reports · 2022Article
- Biological properties and surgical applications of the human amniotic membrane.Frontiers in bioengineering and biotechnology · 2022Review
- A minimally manipulated preservation and virus inactivation method for amnion/chorion.Frontiers in bioengineering and biotechnology · 2022Article
- Placental Tissues as Biomaterials in Regenerative Medicine.BioMed research international · 2022Review
- Proteomic Analysis and Cell Viability of Nine Amnion, Chorion, Umbilical Cord, and Amniotic Fluid-Derived Products.Cartilage · 2021Article
- Exosomes Secreted from Amniotic Membrane Contribute to Its Anti-Fibrotic Activity.International journal of molecular sciences · 2021Article
- Comprehensive Comparison of Amnion Stromal Cells and Chorion Stromal Cells by RNA-Seq.International journal of molecular sciences · 2021Article
- The Role of Collagen-Based Biomaterials in Chronic Wound Healing and Sports Medicine Applications.Bioengineering (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human amniotic membrane (AM) has a long history of clinical use for wound treatment. AM serves as a wound protective barrier maintaining proper moisture. AM is anti-inflammatory, anti-microbial and antifibrotic, and supports angiogenesis, granulation tissue formation and wound re-epithelialization. These properties of AM are attributed to its native extracellular matrix, growth factors, and endogenous cells including mesenchymal stem cells. Advances in tissue preservation have helped to overcome the short shelf life of fresh AM and led to the development of AM products for clinical use. Viable cryopreserved amnion (VCAM), which retains all native components of fresh AM, has shown positive outcomes in clinical trials for wound management. However, cryopreservation requires ultra-low temperature storage and shipment that limits widespread use of VCAM. We have developed a lyopreservation technique to allow for ambient storage of living tissues. Here, we compared the structural, molecular, and functional properties of a viable lyopreserved human amniotic membrane (VLAM) with properties of VCAM using in vitro and in vivo wound models. We found that the structure, growth factors, and cell viability of VLAM is similar to that of VCAM and fresh AM. Both, VCAM and VLAM inhibited TNF-α secretion and upregulated VEGF expression in vitro under conditions designed to mimic inflammation and hypoxia in a wound microenvironment, and resulted in wound closure in a diabetic mouse chronic wound model. Taken together, these data demonstrate that VLAM structural and functional properties are equivalent to VCAM but without the constraints of ultra-low temperature storage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.