ArticleJournal of biomedical optics2018
Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues.
Article in Journal of biomedical optics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Multi-axial strain mapping to characterise structure and material properties of the human hip capsule.PloS one · 2026Article
- Collagen microarchitecture from polarized light imaging: a biomechanics perspective.Journal of biomedical optics · 2026Review
- Multiscale analysis of equatorial sclera anisotropy: Revealing discrepancies in fiber orientation and mechanical properties.Science advances · 2025Article
- Recent progress in the identification and in vitro culture of skin organoids.Regenerative therapy · 2025Review
- Comparing continuum and direct fiber models of soft tissues. An ocular biomechanics example reveals that continuum models may artificially disrupt the strains at both the tissue and fiber levels.bioRxiv : the preprint server for biology · 2024Article
- Direct measurements of collagen fiber recruitment in the posterior pole of the eye.Acta biomaterialia · 2024Article
- 2D or not 2D? Mapping the in-depth inclination of the collagen fibers of the corneoscleral shell.Experimental eye research · 2023Article
- A direct fiber approach to model sclera collagen architecture and biomechanics.Experimental eye research · 2023Article
- Stretch-Induced Uncrimping of Equatorial Sclera Collagen Bundles.Journal of biomechanical engineering · 2023Article
- Instant polarized light microscopy pi (IPOLπ) for quantitative imaging of collagen architecture and dynamics in ocular tissues.bioRxiv : the preprint server for biology · 2023Article
- Morphological Changes of Glial Lamina Cribrosa of Rats Suffering from Chronic High Intraocular Pressure.Bioengineering (Basel, Switzerland) · 2022Article
- Real-time imaging of optic nerve head collagen microstructure and biomechanics using instant polarized light microscopy.Experimental eye research · 2022Article
- Polarization sensitive optical coherence tomography with single input for imaging depth-resolved collagen organizations.Light, science & applications · 2021Article
- Instant polarized light microscopy for imaging collagen microarchitecture and dynamics.Journal of biophotonics · 2021Article
- Self-assembly of differentiated progenitor cells facilitates spheroid human skin organoid formation and planar skin regeneration.Theranostics · 2021Article
- Lamina Cribrosa Capillaries Straighten as Intraocular Pressure Increases.Investigative ophthalmology & visual science · 2020Article
- Quantification of the regional bioarchitecture in the human aorta.Journal of anatomy · 2020Article
- Scleral structure and biomechanics.Progress in retinal and eye research · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Collagen is a major constituent of the eye and understanding its architecture and biomechanics is critical to preserve and restore vision. We, recently, demonstrated polarized light microscopy (PLM) as a powerful technique for measuring properties of the collagen fibers of the eye, such as spatial distribution and orientation. Our implementation of PLM, however, required sectioning the tissues for imaging using transmitted light. This is problematic because it limits analysis to thin sections. This is not only slow, but precludes study of dynamic events such as pressure-induced deformations, which are central to the role of collagen. We introduce structured polarized light microscopy (SPLM), an imaging technique that combines structured light illumination with PLM to allow imaging and measurement of collagen fiber properties in thick ocular tissues. Using pig and sheep eyes, we show that SPLM rejects diffuse background light effectively in thick tissues, significantly enhancing visualization of optic nerve head (ONH) structures, such as the lamina cribrosa, and improving the accuracy of the collagen fiber orientation measurements. Further, we demonstrate the integration of SPLM with an inflation device to enable direct visualization, deformation tracking, and quantification of collagen fibers in ONHs while under controlled pressure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.