ArticleApplications in plant sciences2018
NaOH low-salt method for chloroplast isolation and highly pure cpDNA preparation from
Article in Applications in plant sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 2 citations in OpenAlex.
- Extraction and analysis of high-quality chloroplast DNA with reduced nuclear DNA for medicinal plants.BMC biotechnology · 2024Article
- NaOH low-salt method for chloroplast isolation and highly pure cpDNA preparation fromApplications in plant sciences · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
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Abstract
premise of the studyHigh-yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high-salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochondria. To overcome this issue, we modified a traditional high-salt method to obtain a new approach called the NaOH low-salt method (NLS). METHODS AND
resultsThe NLS method is based on the mild alkaline lysis of plant cells, followed by homogenization with ultrasonic waves and fractionation under reduced osmotic pressure. Results showed that this modified protocol worked efficiently to extract the intact chloroplast from
conclusionsCompared with high-salt methods, the NLS method has proven robust for extraction of intact chloroplasts and preparation of high-yield pure cpDNA from grasses.
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