ArticleNature microbiology2018
In vivo TssA proximity labelling during type VI secretion biogenesis reveals TagA as a protein that stops and holds the sheath.
Article in Nature microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Functional diversity of accessory factors of the type VI secretion system.Journal of bacteriology · 2026Review
- Specific and broad-spectrum antibacterial effectors of type VI secretion system drive competition ofMicrobiology spectrum · 2026Article
- Review
- Phenotypic heterogeneity optimizes trade-offs during adaptive deployment of the type VI secretion system.PLoS biology · 2026Article
- The type VI secretion system and associated effector proteins.Nature reviews. Microbiology · 2026Review
- Tke5 is a Pseudomonas putida toxin that kills plant pathogens by depolarising membranes.Communications biology · 2026Article
- Demonstration of the Role of Both a Ttr and a Psr Homologue Enzymes in the Respiration of Tetrathionate by an Environmental Bacterium Shewanella sp. ANA-3.Environmental microbiology · 2026Article
- The multifunctional type VI secretion system ofApplied and environmental microbiology · 2025Review
- Not just passengers: effectors contribute to the assembly of the type VI secretion system as structural building blocks.Journal of bacteriology · 2025Review
- The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin.Microbial biotechnology · 2025Article
- SspA is a transcriptional regulator of CRISPR adaptation in E. coli.Nucleic acids research · 2025Article
- Exploring Bacterial Surface Proteome Dynamics During Infection Using Proximity Labeling.Methods in molecular biology (Clifton, N.J.) · 2025Article
- BioID in Bacteria: Selection of a Suitable Biotin Ligase for Proxeome Mapping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Functionality of chimeric TssA proteins in the type VI secretion system reveals sheath docking specificity within their N-terminal domains.Nature communications · 2024Article
- Identification of Protein Partners by APEX2 Proximity Labeling.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Exposing the small protein load of bacterial life.FEMS microbiology reviews · 2023Review
- Acinetobacter type VI secretion system comprises a non-canonical membrane complex.PLoS pathogens · 2023Article
- Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.Microbiology (Reading, England) · 2023Review
- Qualitative and Quantitative Methods to Measure Antibacterial Activity Resulting from Bacterial Competition.Bio-protocol · 2023Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The type VI secretion system (T6SS) is a multiprotein weapon used by bacteria to destroy competitor cells. The T6SS contractile sheath wraps an effector-loaded syringe that is injected into the target cell. This tail structure assembles onto the baseplate that is docked to the membrane complex. In enteroaggregative Escherichia coli, TssA plays a central role at each stage of the T6SS assembly pathway by stabilizing the baseplate and coordinating the polymerization of the tail. Here we adapted an assay based on APEX2-dependent biotinylation to identify the proximity partners of TssA in vivo. By using stage-blocking mutations, we define the temporal contacts of TssA during T6SS biogenesis. This proteomic mapping approach also revealed an additional partner of TssA, TagA. We show that TagA is a cytosolic protein tightly associated with the membrane. Analyses of sheath dynamics further demonstrate that TagA captures the distal end of the sheath to stop its polymerization and to maintain it under the extended conformation.
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