Evidence map›Paper›PMID 30275376›Full record

ArticleInternational journal of molecular sciences2018

Highly Efficient Targeted Gene Editing in Upland Cotton Using the CRISPR/Cas9 System.

Shouhong Zhu, Xiuli Yu, Yanjun Li, Yuqiang Sun, Qianhao Zhu, Jie Sun

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Genome-Editing ofPlants (Basel, Switzerland) · 2023
    Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Efficient Genome Editing Using CRISPR/Cas9 Technology in Chicory.International journal of molecular sciences · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shouhong ZhuThe Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi 832000, Xinjiang, China. zhushouhong2014@126.com.
Xiuli YuThe Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi 832000, Xinjiang, China. axiu985211@163.com.
Yanjun LiThe Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi 832000, Xinjiang, China. lyj20022002@sina.com.cn.
Yuqiang SunKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310016, Zhejiang, China. sunyuqiang@zstu.edu.cn.
Qianhao ZhuCSIRO Agriculture and Food, GPO Box 1700, Canberra 2601, Australia. qianhao.zhu@csiro.au.
Jie SunThe Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi 832000, Xinjiang, China. sunjie@shzu.edu.cn.

Funding

Specific Project for Crops Breeding of Shihezi University gxjs-yz03the National Key Research and Development Program of China 2017YFD0101604; 2016YFD0100200the National Natural Science Foundation of China 31760401
6 · The paper itself

Abstract

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) gene editing system has been shown to be able to induce highly efficient mutagenesis in the targeted DNA of many plants, including cotton, and has become an important tool for investigation of gene function and crop improvement. Here, we developed a simple and easy to operate CRISPR/Cas9 system and demonstrated its high editing efficiency in cotton by targeting-ALARP, a gene encoding alanine-rich protein that is preferentially expressed in cotton fibers. Based on sequence analysis of the target site in the 10 transgenic cottons containing CRISPR/Cas9, we found that the mutation frequencies of

Indexed as

Gene EditingBase SequenceCRISPR-Associated Protein 9CRISPR-Cas SystemsGenes, PlantGenetic VectorsGossypiumMutagenesisMutationPlants, Genetically ModifiedRNA, Guide, CRISPR-Cas SystemsCRISPR-Associated Protein 9RNA, Guide, CRISPR-Cas SystemscottonCRISPR/Cas9genome editingGhALARPmutation

Identifiers

PMID30275376
PMCPMC6213220

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.