Evidence map›Paper›PMID 30272996›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2019

Critical role of IL-1α in IL-1β-induced inflammatory responses: cooperation with NF-κBp65 in transcriptional regulation.

Anil K Singh, Sabrina Fechtner, Mukesh Chourasia, Jerry Sicalo, Salahuddin Ahmed

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
  2. Self-Assembly of Anti-Inflammatory Peptide Amphiphiles for Mucosal Health.Chembiochem : a European journal of chemical biology · 2026
    Article
  3. Impact of Erbium and Gadolinium onInternational journal of molecular sciences · 2026
    Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Frontiers in immunology · 2022
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 3 countries.

Anil K SinghDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, Washington, USA.
Sabrina FechtnerDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, Washington, USA.
Mukesh ChourasiaCenter for Computational Biology and Bioinformatics, Amity Institute of Biotechnology, Amity University, Noida, India.
Jerry SicaloDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, Washington, USA.
Salahuddin AhmedDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, Washington, USA.
Washington State University Spokane · USAmity University · IN

Funding

Regulation of IL-6 mediated inflammation and tissue destruction by EGCGR01AR063104 · NIAMS · WASHINGTON STATE UNIVERSITY · PI AHMED, SALAH-UDDIN · 2013 to 2017
$1.7M
NIAMS NIH HHS R01 AR063104
6 · The paper itself

Abstract

The IL-1 cytokines are considered among the first family of cytokines that orchestrate acute and chronic inflammatory diseases. Both IL-1β and IL-1α are members of the IL-1 family; however, their distinct roles in the inflammatory processes remain poorly understood. We explored the role of IL-1α in IL-1β-activated signaling pathways causing synovial inflammation in rheumatoid arthritis (RA). Using synovial fibroblasts isolated from RA joints, we found that IL-1β significantly stimulated IL-1α expression, which was selectively inhibited by blocking the NF-κB pathway. Knockdown of IL-1α using small interfering RNA abolished IL-1β-induced pro-IL-1α and pro-IL-1β expression and suppressed inflammation. Native and chromatin immunoprecipitation studies showed that IL-1α cooperates in NF-κBp65 binding to the distal region of IL-1α promoter and to the proximal region of IL-1β promoter upstream of the transcription start site to stabilize their gene transcription. Molecular dynamics simulation of IL-1α or IL-1β binding to IL-1 receptor showed distinct interaction sites that corroborate with the ability of IL-1α to differentially activate phosphorylation of signaling proteins compared with IL-1β. Our study highlights the importance of IL-1α in mediating IL-1β-induced inflammation in addition to maintaining its expression and providing a rationale for targeting IL-1α to minimize the role of IL-1β in inflammatory diseases like RA.-Singh, A. K., Fechtner, S., Chourasia, M., Sicalo, J., Ahmed, S. Critical role of IL-1α in IL-1β-induced inflammatory responses: cooperation with NF-κBp65 in transcriptional regulation.

Indexed as

Gene Expression RegulationArthritis, RheumatoidCells, CulturedFemaleHumansInflammationInterleukin-1alphaInterleukin-1betaMacrophagesMaleMiddle AgedNF-kappa BPhosphorylationSignal TransductionSynovial MembraneTranscription, GeneticIL1A protein, humanIL1B protein, humanInterleukin-1alphaInterleukin-1betaNF-kappa Bnuclear factor κBrheumatoid arthritissignal transductionsynovial fibroblasts

Identifiers

PMID30272996
PMCPMC6338654
OpenAlexW2893222242

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.