ArticleBiochemistry2018
Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.
Article in Biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Evolution of alcohol oxidase for improved methanol bioconversion and formaldehyde tolerance.Synthetic and systems biotechnology · 2027Article
- Engineered alcohol oxidases catalyse transesterification in aqueous media without competing hydrolysis.Nature communications · 2026Article
- Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases.Synthetic and systems biotechnology · 2025Article
- Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase.Journal of agricultural and food chemistry · 2025Article
- Discovery and biochemical characterization of thermostable glycerol oxidases.Applied microbiology and biotechnology · 2024Article
- Novel Basidiomycetous Alcohol Oxidase fromInternational journal of molecular sciences · 2024Article
- Biosynthetic Strategies of Berberine Bridge Enzyme-like Flavoprotein Oxidases toward Structural Diversification in Natural Product Biosynthesis.Biochemistry · 2024Review
- Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity.Chemical reviews · 2023Review
- Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase.Biochemistry · 2023Article
- Properties, Physiological Functions and Involvement of Basidiomycetous Alcohol Oxidase in Wood Degradation.International journal of molecular sciences · 2022Review
- Three-dimensional structure determination of protein complexes using matrix-landing mass spectrometry.Nature communications · 2022Article
- An Engineered Cholesterol Oxidase Catalyses Enantioselective Oxidation of Non-steroidal Secondary Alcohols.Chembiochem : a European journal of chemical biology · 2022Article
- Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.Biomolecules · 2022Review
- Oxidase enzymes as sustainable oxidation catalysts.Royal Society open science · 2022Review
- Whole-cell screening of oxidative enzymes using genetically encoded sensors.Chemical science · 2021Article
- Directed evolution of alditol oxidase for the production of optically pure D-glycerate from glycerol in the engineered Escherichia coli.Journal of industrial microbiology & biotechnology · 2021Article
- Production of Hydroxy Acids: Selective Double Oxidation of Diols by Flavoprotein Alcohol Oxidase.Angewandte Chemie (International ed. in English) · 2020Article
- Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycerol is a major byproduct of biodiesel production, and enzymes that oxidize this compound have been long sought after. The recently described alcohol oxidase from the white-rot basidiomycete Phanerochaete chrysosporium (PcAOX) was reported to feature very mild activity on glycerol. Here, we describe the comprehensive structural and biochemical characterization of this enzyme. PcAOX was expressed in Escherichia coli in high yields and displayed high thermostability. Steady-state kinetics revealed that PcAOX is highly active toward methanol, ethanol, and 1-propanol ( k
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