ArticleAnalytical chemistry2018
Colorimetric-Luminance Readout for Quantitative Analysis of Fluorescence Signals with a Smartphone CMOS Sensor.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Intraoperative fluorescence imaging with aminolevulinic acid detects grossly occult breast cancer: a phase II randomized controlled trial.Breast cancer research : BCR · 2021Trial
- Smartphone-assisted ratiometric fluorescence sensing platform using Eu/DUT-52 hydrogel for on-site detection of aflatoxin B1.Mikrochimica acta · 2026Article
- Gamma Correction and Color Space Transformations for Quantitative Analysis of Electrochemiluminescence Images Using Smartphone Cameras.Chemical & biomedical imaging · 2025Article
- All-in-One Sustainable Thread Biosensor for Chemiluminescence Smartphone Detection of Lactate in Sweat.Biosensors · 2025Article
- Colorimetric Analysis of Transmitted Light Through Plasmonic Paper for Next-Generation Point-of-Care (PoC) Devices.Biosensors · 2025Article
- Multiplexing LAMP Assays: A Methodological Review and Diagnostic Application.International journal of molecular sciences · 2024Review
- Three-Dimensional-Printed Instrument for Isothermal Nucleic Acid Amplification with Real-Time Colorimetric Imaging.Micromachines · 2024Article
- A Smartphone-Enabled Continuous Flow Digital Droplet LAMP Platform for High Throughput and Inexpensive Quantitative Detection of Nucleic Acid Targets.Sensors (Basel, Switzerland) · 2023Article
- Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics.Sensors (Basel, Switzerland) · 2023Article
- Review
- A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance.The CRISPR journal · 2023Article
- Smartphone-based platforms implementing microfluidic detection with image-based artificial intelligence.Nature communications · 2023Review
- Article
- Computer vision meets microfluidics: a label-free method for high-throughput cell analysis.Microsystems & nanoengineering · 2023Review
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- Diagnosis ofIndian journal of microbiology · 2022Article
- Article
- Virus Detection: From State-of-the-Art Laboratories to Smartphone-Based Point-of-Care Testing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- A handheld-type total integrated capillary electrophoresis system for SARS-CoV-2 diagnostics: Power, fluorescence detection, and data analysis by smartphone.Biosensors & bioelectronics · 2022Article
- Smartphone-enabled field monitoring tool for rapid hexavalent chromium detection in water.Analytical and bioanalytical chemistry · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Smartphones have shown promise as an enabling technology for portable and distributed point-of-care diagnostic tests. The CMOS camera sensor can be used for detecting optical signals, including fluorescence for applications such as isothermal nucleic acid amplification tests. However, such analysis is typically limited mostly to end point detection of single targets. Here we present a smartphone-based image analysis pipeline that utilizes the CIE xyY (chromaticity-luminance) color space to measure the luminance (in lieu of RGB intensities) of fluorescent signals arising from nucleic acid amplification targets, with a discrimination sensitivity (ratio between the positive to negative signals), which is an order of magnitude more than traditional RGB intensity based analysis. Furthermore, the chromaticity part of the analysis enables reliable multiplexed detection of different targets labeled with spectrally separated fluorophores. We apply this chromaticity-luminance formulation to simultaneously detect Zika and chikungunya viral RNA via end point RT-LAMP (Reverse transcription Loop-Mediated isothermal amplification). We also show real time LAMP detection of Neisseria gonorrhoeae samples down to a copy number of 3.5 copies per 10 μL of reaction volume in our smartphone-operated portable LAMP box. Our chromaticity-luminance analysis is readily adaptable to other types of multiplexed fluorescence measurements using a smartphone camera.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.