ArticleJournal of visualized experiments : JoVE2018
Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Morphometric and Biochemical Parameters ofVeterinary sciences · 2026Article
- Protein craving links larval signals to food provisioning in honey bees.Science advances · 2026Article
- Dietary pollen and carbohydrate sources influence longevity, physiology, and gut microbiota in honey bee (Apis mellifera).Scientific reports · 2026Article
- Impact of Direct-Fed Microorganism Syrup on Honey Bee (Animals : an open access journal from MDPI · 2026Article
- Hangry bees: Pollen dearth impacts honey bee (Apis mellifera) behavior and physiology.PloS one · 2026Article
- Organic Acid Supplementation in Worker Honeybees (Insects · 2025Review
- Impact of carbohydrate sources on the longevity and physiological traits of the European honey bee workers.Scientific reports · 2025Article
- Article
- Stress drives premature hive exiting behavior that leads to death in young honey bee (Apis mellifera) workers.Biological research · 2024Article
- Article
- Different effects of pesticides on transcripts of the endocrine regulation and energy metabolism in honeybee foragers from different colonies.Scientific reports · 2023Article
- Developing Strategies to Help Bee Colony Resilience in Changing Environments.Animals : an open access journal from MDPI · 2022Review
- Article
- Honey Bee Genetic Stock Determines Deformed Wing Virus Symptom Severity but not Viral Load or Dissemination Following Pupal Exposure.Frontiers in genetics · 2022Article
- Regulation of dietary intake of protein and lipid by nurse-age adult worker honeybees.The Journal of experimental biology · 2021Article
- Host Genotype and Tissue Type Determine DWV Infection Intensity.Frontiers in insect science · 2021Article
- Exposure to sublethal concentrations of methoxyfenozide disrupts honey bee colony activity and thermoregulation.PloS one · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nurse hypopharyngeal glands produce the protein fraction of the worker and royal jelly that is fed to developing larvae and queens. These paired glands that are located in the head of the bee are highly sensitive to the quantity and quality of pollen and pollen substitutes that the nurse bee consumes. The glands get smaller when nurses are fed deficient diets and are large when they are fed complete diets. Because nurse hypopharyngeal gland size is a robust indicator of nurse nutrition, it is essential that those studying honey bee nutrition know how to measure these glands. Here, we provide detailed methods for dissecting, staining, imaging, and measuring nurse bee hypopharyngeal glands. We present comparisons of unstained and stained tissue and data that were used to study the impact of pollen on gland size. This method has been used to test how diet impacts hypopharyngeal gland size but has further use for understanding the role of these glands in hive health.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.