ArticleJournal of visualized experiments : JoVE2018
Isolation of Cerebral Capillaries from Fresh Human Brain Tissue.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
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- Near-Freezing-Temperature Golgi Neuronal Staining for X-ray Imaging of Human Brain.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Senescent brain cell types in Alzheimer's disease: Pathological mechanisms and therapeutic opportunities.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Review
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- Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis.Fluids and barriers of the CNS · 2024Article
- Oxidative stress alters mitochondrial homeostasis in isolated brain capillaries.Fluids and barriers of the CNS · 2024Article
- Mesenchymal Stem Cell (MSC)-Based Drug Delivery into the Brain across the Blood-Brain Barrier.Pharmaceutics · 2024Review
- Simulating photodynamic therapy for the treatment of glioblastoma using Monte Carlo radiative transport.Journal of biomedical optics · 2024Article
- A high-throughput single-cell RNA expression profiling method identifies human pericyte markers.Neuropathology and applied neurobiology · 2023Article
- Proteasome inhibition protects blood-brain barrier P-glycoprotein and lowers Aβ brain levels in an Alzheimer's disease model.Fluids and barriers of the CNS · 2023Article
- Capillary responses to functional and pathological activations rely on the capillary states at rest.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- β-Estradiol 17-acetate enhances theNeural regeneration research · 2023Article
- A Historical Review of Brain Drug Delivery.Pharmaceutics · 2022Review
- Article
- Viable human brain microvessels for the study of aging and neurodegenerative diseases.Microvascular research · 2022Article
- Article
- Advancing brain barriers RNA sequencing: guidelines from experimental design to publication.Fluids and barriers of the CNS · 2020Review
- Oxidative stress-induced activation of Abl and Src kinases rapidly induces P-glycoprotein internalization via phosphorylation of caveolin-1 on tyrosine-14, decreasing cortisol efflux at the blood-brain barrier.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Article
- Cerebrovascular Senescence Is Associated With Tau Pathology in Alzheimer's Disease.Frontiers in neurology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Understanding blood-brain barrier function under physiological and pathophysiological conditions is critical for the development of new therapeutic strategies that hold the promise to enhance brain drug delivery, improve brain protection, and treat brain disorders. However, studying the human blood-brain barrier function is challenging. Thus, there is a critical need for appropriate models. In this regard, brain capillaries isolated from human brain tissue represent a unique tool to study barrier function as close to the human in vivo situation as possible. Here, we describe an optimized protocol to isolate capillaries from human brain tissue at a high yield and with consistent quality and purity. Capillaries are isolated from fresh human brain tissue using mechanical homogenization, density-gradient centrifugation, and filtration. After the isolation, the human brain capillaries can be used for various applications including leakage assays, live cell imaging, and immune-based assays to study protein expression and function, enzyme activity, or intracellular signaling. Isolated human brain capillaries are a unique model to elucidate the regulation of the human blood-brain barrier function. This model can provide insights into central nervous system (CNS) pathogenesis, which will help the development of therapeutic strategies for treating CNS disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.