ArticleJournal of visualized experiments : JoVE2018
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Red Seaweed-Derived Phycobiliproteins: Marine Bioactive Colorants with Functional Health Properties.Marine drugs · 2026Review
- Photosystem I-independent oxygenic photosynthesis in cyanobacteria.Nature communications · 2026Article
- Low PSI content broadens the optimal light spectrum for a phycoerythrin-dominated cyanobacterium towards near far-red.Scientific reports · 2026Article
- In vitro digestion of cake, biscuit and peanut balls infused with Spirulina using dynamic models.Scientific reports · 2025Article
- Eco-Friendly Extraction to Enhance Antioxidants and Nutritional Value inFoods (Basel, Switzerland) · 2025Article
- Pyridoxine dehydrogenase SePdx regulates photosynthesis via an association with the phycobilisome in a cyanobacterium.The Plant journal : for cell and molecular biology · 2025Article
- Isolation, Identification and Pigment Analysis of Novel Cyanobacterial Strains from Thermal Springs.Plants (Basel, Switzerland) · 2024Article
- A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.Plant physiology · 2024Article
- A Comprehensive Study of Light Quality Acclimation in Synechocystis Sp. PCC 6803.Plant & cell physiology · 2024Article
- Phycobilisome protein ApcG interacts with PSII and regulates energy transfer in Synechocystis.Plant physiology · 2024Article
- Cultivation ofLife (Basel, Switzerland) · 2024Article
- Comparison of antioxidant activity in various spirulina containing products and factors affecting it.Scientific reports · 2023Article
- An extensive review of marine pigments: sources, biotechnological applications, and sustainability.Aquatic sciences · 2023Review
- A Review on a Hidden Gem: Phycoerythrin from Blue-Green Algae.Marine drugs · 2022Review
- Laser Treatment Increases the Antimicrobial Efficacy of Cyanobacterial Extracts againstInternational journal of environmental research and public health · 2022Article
- Trimeric photosystem I facilitates energy transfer from phycobilisomes in Synechocystis sp. PCC 6803.Plant physiology · 2022Article
- Rubisco regulation in response to altered carbon status in the cyanobacterium Synechococcus elongatus PCC 7942.Plant physiology · 2022Article
- Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803.Bioresource technology · 2022Article
- Production of thermostable phycocyanin in a mesophilic cyanobacterium.Metabolic engineering communications · 2021Article
- Effects of Low Temperature, Nitrogen Starvation and Their Combination on the Photosynthesis and Metabolites ofPlants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This is a simple protocol for the quantitative determination of phycobiliprotein content in the model cyanobacterium Synechocystis. Phycobiliproteins are the most important components of phycobilisomes, the major light-harvesting antennae in cyanobacteria and several algae taxa. The phycobilisomes of Synechocystis contain two phycobiliproteins: phycocyanin and allophycocyanin. This protocol describes a simple, efficient, and reliable method for the quantitative determination of both phycocyanin and allophycocyanin in this model cyanobacterium. We compared several methods of phycobiliprotein extraction and spectrophotometric quantification. The extraction procedure as described in this protocol was also successfully applied to other cyanobacteria strains such as Cyanothece sp., Synechococcuselongatus, Spirulina sp., Arthrospira sp., and Nostoc sp., as well as to red algae Porphyridium cruentum. However, the extinction coefficients of specific phycobiliproteins from various taxa can differ and it is, therefore, recommended to validate the spectrophotometric quantification method for every single strain individually. The protocol requires little time and can be performed in any standard life science laboratory since it requires only standard equipment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.